鼻脑通鼻用喷雾剂抑制过氧化氢致SH-SY5Y神经细胞损伤的研究  

Binaotong Inhibits H_2O_2-induced Injury in SH-SY5Y Neuroblastoma Cells

作　　者：侯虹丽[1] 孙芳玲[1] 咸明慧 赵润怀[3] 肖苏萍[3] 艾厚喜[1] 张丽[1] 王文[1] 

机构地区：[1]首都医科大学宣武医院,北京100053 [2]北京祈福瑞草医药科技有限公司,北京100094 [3]中国药材公司,北京100195

出　　处：《中国现代中药》2014年第5期374-379,共6页Modern Chinese Medicine

基　　金："重大新药创制"科技重大专项(2011ZX09102-003-05)

摘　　要：目的:研究鼻脑通对H2O2诱导的人神经母细胞瘤细胞系SH-SY5Y细胞损伤的影响。方法:培养的人神经母细胞瘤细胞系SH-SY5Y细胞给予鼻脑通(0.04,0.4,4μg·mL-1)预孵育24 h后,加入H2O21 000μmol·L-1作用18 h诱导产生损伤,测定细胞上清液中血管活性肽内皮素-1(ET-1)、降钙素基因相关肽(CGRP);炎症因子白细胞介素-1α(IL-1α)、白细胞介素-8(IL-8);致痛物质P物质(SP)、5-羟色胺(5-HT)、一氧化氮(NO)的含量。结果:与对照组相比,损伤组细胞上清液血管活性肽ET-1、CGRP含量显著增多(P<0.01,P<0.05);炎症因子IL-8含量显著增多(P<0.01);致痛物质SP、5-HT、NO含量显著增多(P<0.01,P<0.01,P<0.05)。而与损伤组相比,鼻脑通中、高剂量组细胞上清液血管活性肽ET-1含量降低(P<0.05,P<0.001),且炎症因子IL-8含量也降低(P<0.05,P<0.01);鼻脑通中、高剂量组致痛物质SP含量比损伤组减少(P<0.05,P<0.05),高剂量组5-HT、NO含量比损伤组减少(P<0.05,P<0.05)。结论:鼻脑通治疗偏头痛的机制可能与降低H2O2诱导的SH-SY5Y细胞损伤后血管活性肽ET-1、炎症因子IL-8释放以及抑制致痛物质SP、5-HT、NO的释放有关。Objective： To study the effects of binaotong on H2O2-induced oxidative injury in SH-SY5Y neuroblastoma cells. Methods： SH-SY5Y cells were pre-incubated with binaotong（ 0. 04,0. 4,4 μg·mL- 1） for 24 h prior to exposure to H2O2（ 1 000 μmol·L^- 1） for 18 h. The content of vasoactive petide endothelin-1（ ET-1）, calcitonin gene-related peptide（ CGRP）; inflammatory cytokines interleukin-1α（ IL-1α）,interleukin-8（ IL-8）; pain producing substance substance P（ SP）,5-hydroxy-tryptamine（ 5-HT）,nitric oxide（ NO） were determined through supernatant. Results： Pretreatment the cells with binaotong（ 0. 04,0. 4,4 μg·mL- 1）,supernatant of each injury group of vasoactive petide ET-1,CGRP were increased significantly compared with control group（ P 〈0. 01,P 〈0. 05）; supernatant of injury group of inflammatory cytokine IL-8 was increased significantly compared with control group（ P〈0. 01）;supernatant of each injury group of pain producing substance SP,5-HT,NO were increased compared with control group（ P〈0. 01,P 〈0. 01,P〈0. 05）. However,supernatant vasoactive petide ET-1 level was decreased in the binaotong treated group with middle dose and high dose（ P 〈0. 05,P 0. 001）,and supernatant inflammatory cytokine IL-8 level was also decreased（ P〈0. 05,P 〈0. 01）; supernatant pain producing substance SP level was decreased in the binaotong treated group with middle dose and high dose（ P 〈0.05,P 〈0.05）,supernatant pain producing substance5-HT,NO level were decreased in the binaotong treated group of high dose（ P 〈0. 05,P 〈0. 05）. Conclusion： Decreased supernatant vasoactive petide ET-1 level and inflammatory cytokine IL-8 level and pain producing substance SP,5-HT,NO levels after H2O2-induced injury in SH-SY5Y neuroblastoma cells were the possible mechanisms of binaotong in treating migraine.

关 键 词：鼻脑通 SH-SY5Y神经细胞 ET-1 CGRP IL-1Α IL-8 SP 5-HT NO 

分 类 号：R995[医药卫生—毒理学]