基于线粒体DNA标记的长江中游草鱼亲本增殖放流的遗传效果评估  

Genetic effects of released mature Ctenopharyngodon idellus on natural populations based on the mitochondrial DNA markers in the middle reaches of the Yangtze River

作　　者：李树华[1,2] 陈大庆[2] 段辛斌[2] 刘绍平[2] 汪登强[1] 

机构地区：[1]华中农业大学水产学院,武汉430070 [2]中国水产科学研究院长江水产研究所,武汉430223

出　　处：《淡水渔业》2014年第3期45-50,共6页Freshwater Fisheries

基　　金：公益性行业(农业)科研专项经费(200903048-01)

摘　　要：为探索草鱼(Ctenopharyngodon idellus)亲本增殖放流对野生群体遗传多样性和遗传结构的影响,对2011和2012年在长江中游增殖放流的4个草鱼亲本群体和当年草鱼早期资源调查的2个野生群体共182个个体的线粒体Dloop和Cytb基因片段进行遗传分析。结果发现,在2 046 bp的DNA片段中共发现65个多态位点,22个简约信息位点,定义了39个单倍型,群体平均单倍型多样性(Hd)为0.772,平均核苷酸多样性(π)为0.001 00,表明六个草鱼群体的遗传多样性较低,且群体间无差异。群体间的分子方差分析(AMOVA)显示群体间遗传分化系数为FST=0.010 12,P>0.05,差异不显著,表明群体间不存在遗传分化。混合群体的鉴定分析表明,两个野生群体在混合群体内的比例分别为48.914 5%和27.054 5%。研究结果说明当前增殖放流草鱼亲本对野生群体的遗传多样性和遗传结构没有造成影响。In order to assess the effects of genetic diversity and genetic structure of released parent Ctenopharyngodon idellus on natural populations in the middle of Yangtze River,four released mature C. idellus populations and two natural populations of captured junior in the middle Yangtze River in 2011 and 2012 were studied based on mitochondrial Cyt b and Dloop sequences. The results showed that 65 polymorphic sites in the 2 046 bp aligned sequences,and 39 different haplotypes were identified among 182 samples. The average haplotype diversity（ Hd） and the average nucleotide diversity（ π） of six populations were 0. 772 and 0. 001 0,respectively,revealing low genetic diversity. The genetic differential index FSTby analysis of molecular variance（ AMOVA） among the six populations was 0. 010 12,which meaned that there were no genetic differentiation among populations（ P〉 0. 05）. Mixed stock identification analyses indicated that the proportions of two natural populations（ ZBC,ZCC） in the mixed population were 48. 914 5% and 27. 054 5%,respectively. So it now should be little impacts of genetic diversity and genetic structure by released mature C. idellus on natural populations in this study.

关 键 词：草鱼(Ctenopharyngodon idellus) 增殖放流 遗传多样性 遗传结构 线粒体DNA 

分 类 号：S931.5[农业科学—渔业资源]