乌鲁木齐10号泉水体可培养细菌群落的季节动态变化  

Seasonal Dynamics of Culturable Bacterial Community at No.10 Spring in Urumqi

作　　者：罗娇[1,2] 李萍[3] 薛娟[1] 尤陆花 高小其[4] 杨红梅[2] 

机构地区：[1]石河子大学生命科学学院,新疆石河子832003 [2]新疆农业科学院微生物应用研究所,乌鲁木齐830091 [3]新疆大学生命科学与技术学院,乌鲁木齐830046 [4]新疆维吾尔自治区地震局,乌鲁木齐830011

出　　处：《新疆农业科学》2014年第3期517-523,共7页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区高技术研究发展项目(201216145)

摘　　要：【目的】了解乌鲁木齐10号泉水体可培养细菌群落的季节变化及各类群对水文理化指标的响应。【方法】利用平板菌落计数方法,监测2012年1月至2013年1月期间10号泉水体可培养细菌菌落数目;结合16S rRNA基因序列系统分析法,分析可培养细菌类群组成;采用典范对应分析(CCA),探讨泉水水文理化指标对可培养细菌类群的影响。【结果】调查期内乌鲁木齐10号泉水体可培养细菌菌落数目总体较少,其中与其它季节相比秋季菌落数目相对较多;水体可培养菌株序列分析表明,考克氏菌属、沙雷氏菌属、嗜血杆菌属、假单胞菌属、动性球菌属、芽孢杆菌属、肉食杆菌属和金黄杆菌属较丰富;典范相关分析(CCA)显示,水体可培养细菌类群与He、CH4、HS-、流量成正相关,并受多种水文理化指标共同作用。【结论】从季节变化看,乌鲁木齐10号泉水体可培养细菌数量基本变化趋势是秋季>夏季>春季>冬季。可培养细菌类群可对部分水文理化指标的变化产生响应。[Objective] The aim of this study was is to investigate dynamics of the culturable culturable bacterial number with seasons change at the No.10 Sspring in Urumqi,and the response to hydrological physical and chemical indicators.[Method] The seasonal dynamics of culturable bacterial numbers were detected using culture method from January 2012 to January 2013.The culturable bacterial community composition was examined by 16S rRNA phylogenetic classification.And the correlations between hydrological physical and chemical indicators were analyzed by canonical correspondence analysis （CCA）.[Result] Statistics showed that the culturable bacterial number was low throughout the year,and the bacterial population in No.10 spring water was higher in the autumn than in any other seasons.Sequence analysis showed that Kocuria,Serratia,Haemophilcs,Pseudomonas,Planococcus,Bacillus,Carnobacterium and Chryseobacterium were relatively abundant bacteria communities.The canonical correspondence analysis showed that water culturable bacterial communities have positive correlation with hydrological physical and chemical indicators such as He,CH4,HS-,and flow.[Conclusion] On the seasonal scale,the total average numbers of culturable bacteria showed high values in autumn,followed by summer and spring,with the lowest contents in winter.And the culturable bacterial communities could response respond to part of the hydrogeochemical parameter changes.

关 键 词：泉水 可培养细菌 水文理化指标 动态变化 

分 类 号：Q93[生物学—微生物学]