四倍体刺槐茎段组织石蜡切片的制作方法  

Preparation of Paraffin Section of Tetraploid Robinia pseudoacacia Stem Segment Tissue

作　　者：权金娥[1] 朱海兰[1] 张春霞[1] 张胜[1] 赵忠[1] 

机构地区：[1]西北农林科技大学西部环境与生态教育部重点实验室,陕西杨陵712100

出　　处：《西北林学院学报》2014年第3期140-144,共5页Journal of Northwest Forestry University

基　　金：国家自然科学基金项目"四倍体刺槐生根调控机理研究"(30972352)

摘　　要：以传统石蜡切片方法为基础,针对林木茎段木质化程度高、硬度和脆性大的特点对固定、软化、脱水、透明、染色等具体步骤进行了改进,并成功应用于四倍体刺槐生根过程插穗基部解剖学研究。结果表明:较传统方法切片时组织不易碎,切片结构完整,减少了脱水时间,缩短了制片周期;获得了厚度为8μm且染色清晰、细胞结构完整的林木茎段横切面切片;四倍体刺槐插穗石蜡切片可清晰的观察大量能够反映生根各时期发育特征。该方法为进一步从解剖学上研究林木茎段组织生长机制提供基础,为探究其组织形态变化提供了新的技术方法。Traditional method of making paraffin section was modified from the aspects of fixing,softening, dehydration,transparency,dyeing and other steps to fit the hard,crisp and highly lignified woody stem segment.This modified method was successfully applied to observe the rooting process of cuttings of Robinia pseudoacaciatetraploid.We made a 8μm cross-section slice with clearly staining and complete organizational structure.The method reported in this study would provide theoretical and technical basis for anatomical observation to study the growing mechanism of woody plants as well as for identification of histomorphology changes.

关 键 词：四倍体刺槐 林木茎段 石蜡切片 制作方法 

分 类 号：S792.27[农业科学—林木遗传育种]