大肠杆菌生物膜毒力基因的检测  

Checking Virulence Genes of Biofilm in Escherichia coli

作　　者：喻华英[1] 张亮亮[1] 

机构地区：[1]新疆塔里木大学动物科学学院,新疆阿拉尔843300

出　　处：《中国农学通报》2014年第8期17-22,共6页Chinese Agricultural Science Bulletin

基　　金：国家自然基金"新疆地区犊牛腹泻病中大肠杆菌基因型分型及bfpA基因生物学功能探讨"(31060344)

摘　　要：通过确定大肠杆菌形成生物膜毒力基因的分布情况,为大肠杆菌生物膜的形成机理提供理论依据。本实验利用PCR聚合酶链式反应,对25株大肠杆菌阳性菌株菌进行了相关毒力因子fimH,KpsMTII,chuA,fliC,yja和PapC的检测筛选。其结果显示:KpsMTII在所检测25株菌中出现的频率最高,占40%;其次是fliC,占32.5%,fimH占20%,合计占52.5%;chuA占7.5%,yja和PapC均未检出。结果表明:细菌粘附结构fimH基因和fliC基因是影响大肠杆菌形成生物膜的主要粘附结构毒力基因,其次是大肠杆菌毒力因子KpsMTII基因,最后是外膜蛋白血红素chuA基因。The test was aimed of confirmed these genes distribution condition in positive biofilm with 25 strains of Escherichia coli, and then provided the theoretical basis of the mechanism of biofilm during the pathopoiesia in the Escherichia coli. The polymerase chain reaction was used of being checked virulence genes in the fimH, KpsMTII, chuA, fliC, yja and PapC in 25 strains of Escherichia coli which were positive biofilm. The results showed that, Firstly, the gene of KpsMTII aroused frequence was higjhest which account of 40%; Secondly that the gene of fliC account of 32.5% and the gene of fimH account of 20%, the two genes total was 52.5%; the gene of chuA account of 7.5 %, the genes of yja and PapC unchecked in this test. In this test the biofilm formed were firstly effected in adhere structure genes such as fimH and fliC. Secondly effected gene was KpsMTII, Finally, effected gene was outer membrane protein gene chuA in 25 strains Escherichia coli.

关 键 词：生物膜 毒力基因 检测 

分 类 号：S852.612[农业科学—基础兽医学]