生防细菌对植物根围微生态效应的研究进展  

Advances on Micro-ecological Effects of Antagonistic Bacteria on Rhizosphere

作　　者：刘丹[1,2] 张丽萍[2] 史延茂[2] 尹淑丽[2] 崔少飞[1,2] 

机构地区：[1]河北工业大学化工学院,天津300130 [2]河北省科学院生物研究所,石家庄050081

出　　处：《中国农学通报》2014年第7期260-265,共6页Chinese Agricultural Science Bulletin

基　　金：国家星火计划项目"畜禽粪便资源化利用技术集成与示范"(2012GA6200022);河北省重点基础研究项目"枯草芽胞杆菌BSD-2及其抗菌素防治蔬菜灰霉病作用机理研究"(13966503D);河北省科学院重大支撑项目"绿色蔬菜生产技术体系研究与示范"(13301)

摘　　要：利用生防细菌防治植物土传病害是农业可持续发展的趋势,研究生防细菌对根围微生态的影响对生防细菌防治作用的有效发挥及安全合理使用具有重要指导意义,为评估生防细菌的生态安全提供重要依据。本研究介绍了传统的分离培养、BIOLOG微平板、PLFA以及基于PCR技术的植物微生态学的研究方法,从生防细菌亲缘关系较近的细菌、微生物群落结构及根围土壤酶活3个方面对生防细菌产生的根围微生态影响进行总结,并对未来的研究方向进行了展望。It is a trend of agricultural persistence development that prevention and control of the plant soilborne diseases using antagonistic bacteria. The investigation of the effects of antagonistic bacteria on rhizosphere micro-ecology has important guiding significance for its effective protection and secure application, which can also provide crucial evidences for evaluating the environmental security of antagonistic bacteria. This paper reviewed the research method of plant micro-ecology, such as cultivation techniques, BIOLOG microplate techniques, PLFA method and PCR-based methods, and summarized the micro-ecology effect of antagonistic bacteria on rhizosphere, including the effect on closely related bacteria, the community structure of microorganism and the enzyme activity of rhizosphere soil. Furthermore, the future research direction was prospected.

关 键 词：生防细菌 根际 微生态 

分 类 号：S476[农业科学—农业昆虫与害虫防治]