Hg^(2+)、Cu^(2+)、Zn^(2+)、Cd^(2+)胁迫下罗非鱼离体肝脏中GSH和GST的响应  

Responses of GSH and GST in liver of Mossambica tilapia under Hg^(2+),Cu^(2+),Zn^(2+) and Cd^(2+) stress in Vitro

作　　者：肖丹[1] 王海燕[2] 韩大雄[1] 

机构地区：[1]厦门大学药学院,福建厦门361005 [2]国家海洋局第三海洋研究所,福建厦门361005

出　　处：《海洋环境科学》2014年第3期346-350,共5页Marine Environmental Science

基　　金：海洋公益性项目(201105013);福建省重大专项/专题项目(2011YZ0001-1);国家海洋局第三海洋研究所基本科研业务费专项基金资助项目(201221);福建省自然科学基金项目(2013J01386)

摘　　要：以罗非鱼(Mossambica tilapia)为实验材料,研究了不同浓度的4种金属离子(Hg2+、Cu2+、Zn2+、Cd2+)体外暴污对其肝脏上清液中谷胱甘肽(GSH)含量和谷胱甘肽-S-转移酶(GST)活性的影响,以探讨其用于污染暴露的生物标记的可能性。结果表明:暴露于上述4种金属离子的罗非鱼肝上清液中,GSH含量均为低浓度升高,高浓度降低。Hg2+、Cu2+、Zn2+处理组均在100 nmol/L时,GSH含量上升至最大值,与对照组相比,均差异极显著(p≤0.01)。GST的活性则均随着4种金属离子浓度的升高总体呈下降趋势,4个处理组GST活性的变化在整个浓度范围内的剂量-效应关系均存在一定的线性规律。实验结果表明鱼类肝上清液中GSH含量和GST活性的变化很有潜力运用于环境监测系统中,成为环境中金属离子污染的早期生物预警标志物。This paper approached the feasible of using the GSH content and GST activity in Mossambica tilapia liver supernatant as the biological indicator of 4 heavy metals（Hg^2 ＋,Cu^2 ＋,Zn^2 ＋,Cd^2 ＋） toxic effect on this fish. Results indicated that the GSH content was elevated by low concentration of the 4 heavy metals while reduced by high dosage of the 4 pollutants. When the concentration of Hg^2 ＋,Cu^2 ＋,Zn^2 ＋was 100 nmol / L,the GSH content increased significantly and reached the maximum（p≤0. 01）. The activity of GST decreased continuously as the concentration of 4 heavy metals increased and there was a certain dose-response linear relationship in the whole assayed concentrations. The results indicated that the change of GSH content and GST activity in the fish liver supernatant could be a potential biomarker,which could be used in environmental monitoring system to reflect the early heavy metal ion pollution.

关 键 词：罗非鱼 重金属 谷胱甘肽 谷胱甘肽-S-转移酶 肝上清液 

分 类 号：Q178.53[生物学—水生生物学]