RP-HPLC法同时测定旱柳叶药材中3个主要黄酮苷成分的含量  

RP-HPLC simultaneous determination of three main flavonoid glycoside components in Hankow Willow Leaf

作　　者：左飞鸿 吴国江 李进进 周国平 付辉政 郭文 

机构地区：[1]江西国药有限责任公司,南昌330052 [2]江西省食品药品检验所,南昌330046

出　　处：《药物分析杂志》2014年第5期903-906,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的：建立同时测定旱柳叶药材中木犀草苷、芹菜素-7-O-β-D-吡喃葡萄糖醛酸苷和木犀草素-3’-甲醚-7-O-β-D-吡喃葡萄糖醛酸苷含量的高效液相色谱法。方法：采用YMC-Pack ODS-A（150 mm×4.6 mm,5μm）色谱柱,以乙腈-0.2%磷酸溶液（15∶85）为流动相,流速1.0 mL·min-1,检测波长344 nm。结果：木犀草苷,芹菜素-7-O-β-D-吡喃葡萄糖醛酸苷和木犀草素-3’-甲醚-7-O-β-D-吡喃葡萄糖醛酸苷进样量分别在0.26～2.08μg（r=0.9999）、0.30～2.40μg（r=0.9999）和0.62～4.96μg（r=0.9999）范围内与峰面积呈良好线性关系;平均回收率（n=6）分别为97.5%、98.8%和101.5%,RSD分别为1.7%、1.7%和1.6%。结论：经方法学验证,本法可用于旱柳叶药材的质量控制。Objective： To establish a method for the simultaneous determination of the contents of luteolin-7-O-β-D-glucoside,apigenin-7-O-β-D-pyranglycuronide and lutelion-3＇-methylether-7-O-β-D-pyranglycuronide in Hankow Willow Leaf by HPLC. Methods： The separation was performed on a YMC-Pack ODS-A column（ 150 mm × 4. 6 mm,5 μm） with a mobile phase of acetonitrile-0. 2% phosphoric acid solution（ 15∶ 85）. The flow rate was 1. 0 mL·min-1,and the detecting wavelength was 344 nm. Results： The linear ranges of luteolin-7-O-β-D-glucoside,apigenin-7-O-β-D-pyranglycuronide and lutelion-3＇-methylether-7-O-β-D-pyranglycuronide were 0. 26-2. 08 μg（ r = 0. 9999）,0. 30-2. 40 μg（ r = 0. 9999） and 0. 62-4. 96 μg（ r = 0. 9999）,respectively. The average recoveries（ n = 6） were 97. 5%（ RSD = 1. 7%）,98. 8%（ RSD =1. 7%） and 101. 5%（ RSD =1. 6%）,respectively. Conclusion： Based on method validation,the method can be used for quality control of the leaves of Salix matsudana Koidz.

关 键 词：旱柳叶 木犀草苷 芹菜素-吡喃葡萄糖醛酸苷 木犀草素-甲醚-吡喃葡萄糖醛酸苷 反相高效液相色谱法 方法学验证 中药材质量控制 

分 类 号：R917[医药卫生—药物分析学]