HPLC法测定5-氟尿嘧啶纳米粒的药物包封率  

HPLC determination of entrapment efficiency of 5-fluorouracil nanoparticles

作　　者：赵暖暖[1] 仵文英[2] 张抗怀[2] 王娜[2] 

机构地区：[1]西安交通大学医学院药学系,西安710061 [2]西安交通大学医学院第二附属医院药剂科,西安710004

出　　处：《药物分析杂志》2014年第5期939-942,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的：制备5-氟尿嘧啶（5-FU）纳米粒,并采用高效液相色谱法测定纳米粒中5-FU的包封率。方法：采用凝胶柱过滤分离5-FU纳米粒中的游离药物,以HPLC为分析方法对纳米粒包封率进行测定。色谱条件：采用Agilent C18柱（250 mm×4.6 mm,5μm）,以甲醇-水（10∶90）为流动相,流速1.0 mL·min-1,检测波长266 nm,柱温30℃,进样量为20μL。结果：5-FU峰与溶剂峰分离良好,5-FU浓度在2～14μg·mL-1范围内线性关系良好（r=0.9998）;平均回收率在96.5%～97.8%之间。结论：该方法经方法学验证,可适用于5-FU纳米粒中药物包封率的测定。Objective： To prepare 5-fluorouracil（ 5-FU） nanoparticles and establish an HPLC method for the determination of 5-FU. Methods： Free 5-FU（ non-associated to the nanostructures） was separated from the nanoparticles by Sephadex gel,with HPLC on analysis for determination of entrapment efficiency of 5-FU nanoparticles. An Agilent C18column（ 250 mm × 4. 6 mm,5 μm） was used,and methanol-water（ 10∶ 90） was used as the mobile phase. The flow rate was 1. 0 mL·min-1; the detection wavelength was 266 nm; the sample volume was 20 μL and the column temperature was 30 ℃. Results： the peak of 5-FU and solvent could be separated well. The linear range of 5-FU was 2-14 μg·mL-1（ r =0. 9998）. The average recovery was 96. 5%-97. 8%. Conclusion： This method is suitable for the assay of entrapment efficiency of 5-FU nanoparticles.

关 键 词：5-氟尿嘧啶(5-FU) 聚己内酯 纳米粒 包封率 凝胶过滤 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]