应用胎盘组织芯片检测子痫前期中E-cadherin的表达  

Detection of E-cadherin expression in preeclampsia by placenta tissue microarray

作　　者：张展[1] 李伟[1] 张琳琳[1] 贾莉婷[1] 于海洋[1] 刘丽莎[1] 

机构地区：[1]郑州大学第三附属医院检验科,河南郑州450052

出　　处：《中国卫生检验杂志》2014年第9期1232-1235,共4页Chinese Journal of Health Laboratory Technology

摘　　要：目的通过应用胎盘绒毛滋养细胞(VCT)和绒毛外滋养细胞(EVCT)组织芯片来检测子痫前期(PE)患者Ecadherin的表达水平,探讨E-cadherin在PE发病中的作用。方法免疫组织化学技术(IHC)检测胎盘VCT组织芯片(子痫前期56例,正常对照组42例)和EVCT组织芯片(子痫前期47例,正常对照组29例)中E-cadherin的表达情况。结果 E-cadherin主要表达于胎盘合体滋养层细胞和EVCT的细胞膜上,E-cadherin蛋白在PE组胎盘VCT和EVCT组织芯片中的表达水平明显高于正常对照组(χ2=16.696,P=0.000;χ2=16.735,P=0.000)。结论 E-cadherin在胎盘组织表达增加可能与子痫前期的发病相关。Objective To investigate the possible pathophysiological importance of E- cadherin in preeclampsia（ PE） by the application of placenta villous cytotrophoblast（ VCT） and extravillious cytotrophoblast（ EVCT） tissue microarray. Methods Immunohistochemistry methods were used to detect the expression of E- cadherin on VCT tissue microarray（consisting of 56 PE cases,42 normal controls） and EVCT tissue microarray（consisting of 47 PE cases,29 normal controls） respectively. Results The E- cadherin protein was found mainly in the cytomembrane of the villous syncytiotrophoblast and the EVCT. Compared with the normal control group,the E- cadherin showed a stronger staining intensity in PE group（ χ^2= 16. 696,P = 0. 000; χ^2= 16. 735,P = 0. 000）. Conclusion The increased placental expression of E- cadherin may be associated with pathogenesis of PE.

关 键 词：子痫前期 E-CADHERIN 绒毛滋养细胞 绒毛外滋养细胞 组织芯片 

分 类 号：R714.244[医药卫生—妇产科学]