量子点偶联RGD用于喉癌血管的靶向活体成像  

Quantum Dot Conjugated RGD for Targeted in Vivo Imaging of Laryngocarcinoma Vessel

作　　者：朱小妹[1] 王晓梅[2] 冯刚[2] 陈强[1,2] 林桂淼[2] 赵郡婷 许改霞[1] 牛憨笨[1] 

机构地区：[1]深圳大学光电工程学院教育部/广东省光电子器件与系统重点实验室,广东深圳518060 [2]深圳大学医学院深圳市生物医学工程重点实验室,广东深圳518060

出　　处：《中国激光》2014年第5期97-102,共6页Chinese Journal of Lasers

基　　金：国家973计划(2012CB85802);国家自然科学基金(61235012;61335001);广东省科技创新项目(2012KJCX0094)

摘　　要：研究了偶联环状精氨酸-甘氨酸-天冬氨酸-D-苯丙氨酸-赖氨酸[c(RGDfK)]肽段的CdSe/ZnS量子点(QDRGD)对喉癌血管靶向成像。利用羧基与氨基反应将c(RGDfK)肽段与QD偶联;采用荧光分光光度计对QD-RGD在RMPI1640培养基和小鼠血清溶液中的光谱稳定性进行了检测;利用荧光显微镜检测QD-RGD对Hep-2细胞和MCF-7细胞上整合素αvβ3的靶向性;最后将QD-RGD尾静脉注射到小鼠体内,检测了其对皮脊翼视窗中喉癌血管的靶向性。结果表明QD-RGD的发射光谱在RMPI1640培养基4h内没有明显变化,在小鼠血清中24h内发射光谱的荧光强度仅下降了20%;对细胞荧光成像表明QD-RGD能特异性与细胞表面的整合素αvβ3结合;血管成像表明QD-RGD在注射2h后聚集在喉癌局部血管,24h后QD-RGD从血管中移除。该研究表明QD-RGD能用于活体喉癌肿瘤血管靶向成像,这为喉癌的靶向诊断和靶向治疗研究提供了参考。The targeted imaging of quantum dot （QD） conjugated cyclo （Arg-Gly-Asp-D-Phe-Lys） peptides [c （RGDfK）, QD-RGD] for laryngeal cancer blood vessel in vivo is studied. QD is conjugated with c（RGDfK） peptides by the reaction of carboxyl and amino groups. The spectra stabilities of QD-RGD in RMPI1640 and mouse serum are measured by fluorescence spectrophotometer. The targeting of QD-RGD to avβ3 on Hep-2 and MCF-7 cells is studied by fluorescent microscope. Finally, the targeting of QD-RGD to laryngeal cancer vascular in dorsal skin fold window chamber by tail intravenous injection is investigated. The result shows that the spectra stability of QD-RGD in RPMI1640 does not obviously change in 4 hours. The fluorescence intensity of QD-RGD in mouse serum in 24 hours only decreases by 20 %. The result of cells fluorescence imaging shows that QD-RGD can specifically bind to integrin a1β1 on cells. The result of vascular imaging shows QD-RGD gathers in cancer blood vessel after injecting for 2 hours, and QD-RGD is removed from cancer blood vessel after 24 hours. The study demonstrates that QD-RGD can be used to targeted image cancer blood vessel in vivo, which offers a reference for studying targeting diagnosis and targeting therapy of laryngocarcinoma in vivo.

关 键 词：医用光学 量子点 环状精氨酸-甘氨酸-天冬氨酸-D苯丙氨酸-赖氨酸肽段 整合素av β3 皮脊翼视窗 肿 瘤血管 

分 类 号：Q632[生物学—生物物理学]