Y染色体长度异常与精子凋亡和生殖结局相关性研究  

The relationship of abnormal lengthy Y chromosome with sperm apoptosis and reproductive outcome

作　　者：彭亮[1,3] 吴昕[2,3] 马强[1,3] 贾韶彤[2,3] 景万红[2,3] 阳清虎[2,3] 刘春莲[2,3] 

机构地区：[1]宁夏医科大学基础医学院实验中心,银川750004 [2]宁夏医科大学总医院生殖医学中心 [3]宁夏医科大学生育力保持教育部重点实验室

出　　处：《中国男科学杂志》2014年第4期27-30,共4页Chinese Journal of Andrology

基　　金：宁夏自然科学基金面上项目（NZ11281）;宁夏医科大学面上科研项目（XQ2011020）

摘　　要：目的：探讨辅助生殖技术过程中，Y染色体长度异常的影响机制，分析Y染色体长度异常与精子凋亡和生殖结局的相关性。方法将研究对象分为A组（大Y组），B组（小Y组）以及C组（对照组），分别为28例、16例及30例。采用磷脂结合蛋白V（Annexin V）/碘化丙啶（Propidium iodide, PI）精子染色，通过流式细胞仪（FCM）检测精子亚群所占的比例。结果（1）男性年龄、精液量、密度、活率以及AN-/PI-在3组中分布无差异；B组与C组相比，AN＋/PI-和AN/PI＋有统计学意义（P＜0.05）；（2）以AN＋/PI-值为20%分组，A，B，C组受精率、临床妊娠率以及早期胚胎丢失率在2组中均无统计学差异；但AN＋/PI-≥20%，B组与C组相比，临床妊娠率有统计学差异（P＜0.05）。结论大Y染色体与精子凋亡无明显相关性，小Y可能与精子凋亡相关并影响生殖结局。Objective To explore the effects of abnormal lengthy Y chromosome on reproductive outcome and sperm apotosis and analyze their relationship. Methods The object of study were divided into three groups including Group A （big Y chromosome）, Group B （small Y chromosome） and Group C （controls）. DNA fragmentation was stained by Annexin V/PI assay,the results were analyze by flow cytometry （FCM）to determine the ratio of AN^-/PI^-, AN^＋/ PI^- and AN/PI^＋. Results （1）No significant differences were found in male age ,sperm volume, parameters and AN^-/PI^- among three groups;there were significant difference in AN^＋/PI^- and AN/PI^＋ between Group B and Goup C （P〈0.05）. （2）There was no changes in fertilization rate, clinical pregnancy rate and early pregnancy loss rate among three groups; however, for AN^＋/PI^-≥20% ,implantation rate was significantly differece between group B and group C （P〈0.05）. Conclusion Big Y chromosome had no correlation with sperm apoptosis, while small Y chromosome may be related with sperm apoptosis and reproductive outcome.

关 键 词：染色体  人  Y 精子 细胞凋亡 生殖结局 

分 类 号：R321.33[医药卫生—人体解剖和组织胚胎学]