人ERCC1基因启动子荧光素酶报告基因质粒的构建  

Construction of the Human ERCC1 Luciferase Reporter Plasmid Promoter

作　　者：宋莹[1] 谷依学[1] 仇秦威[1] 贺智敏[1] 

机构地区：[1]广州医科大学附属肿瘤医院,广东广州510095

出　　处：《中外医学研究》2014年第14期144-145,共2页CHINESE AND FOREIGN MEDICAL RESEARCH

摘　　要：目的:构建人切除修复交叉互补基因1(excision repair cross complementation 1,ERCC1)启动子荧光素酶报告基因质粒。方法:以人基因组DNA为模板,扩增ERCC1启动子,将其重组到荧光素酶报告基因载体pGL4 Basic中,测序验证构建的质粒中包含ERCC1启动子序列。结果:测序结果正确,质粒pGL4-ERCC1-P1构建成功。结论:人ERCC1启动子荧光素酶报告基因质粒构建成功,可以用于后续基因表达调控的研究。Objective：To construct a human excision repair cross complementation 1（ERCC1） promoter luciferase reporter plasmid.Method：The ERCC1 promoter from human genomic DNA was amplified by PCR,and was inserted into the pGL4 Basic vector.The amplified DNA sequence was confirmed by sequencing.Result：DNA sequencing verified the successful construction of the plasmid pGL4-ERCC1-P1.Conclusion：The human ERCC1 promoter luciferase reporter gene vector is successfully constructed,which can be use for further study on gene expression regulation.

关 键 词：报告基因 ERCC1 启动子 

分 类 号：R346[医药卫生—基础医学]