高效效相色谱法测定人血清甲氨蝶呤的浓度  

Determination of methotrexate concentration in human serum by HPLC

作　　者：宋新文[1] 汪洋[1] 刘炘[1] 许琼[1] 彭静[1] 

机构地区：[1]武汉市妇女儿童医疗保健中心药学部,武汉430016

出　　处：《中国临床药理学杂志》2014年第5期448-450,共3页The Chinese Journal of Clinical Pharmacology

摘　　要：目的建立一种快速、准确测定人血清中甲氨蝶呤浓度的高效液相色谱法。方法色谱柱为HypersilC。流动相为甲醇一乙腈一磷酸盐缓冲液（2：7：91），流速为0．8mL·min-1，检测波长313nm；血清样品上SPE柱，以含甲醇一乙腈一磷酸盐缓冲液（50：50：250）为洗脱剂，收集洗脱液直接进样测定。结果甲氨蝶呤血清样品线性范围为0．03—50．00μg·mL～，2组低、中、高不同浓度（A组：3．41，6．82，27．27μg·mL～；B组：0．05，0．21，0．85μg-mL。）方法回收率均大于97％。日内、日间RSD均小于5％。结论本方法准确性好、灵敏度高、操作简便，完全可满足儿科临床甲氨蝶呤血药浓度监测需要。Objective To establish for determination the concentration Methods Chromatographic column a rapid and accurate HPLC method of methotrexate in human serum. was Hypersil Cl8 column; mobile phase was methanol,acetonitrile and phosphate buffer （2：7：91 ）, a flow rate was 0. 8 mL · min- 1 ; detection wavelength was 313 nm. Serum sam- ple was injected into SPE column. Methanol/acetonitrile/phosphate buff- er （50： 50： 250） was used as the eluent, and the collection of eluent was detected directly. Results The linear range of methotrexate in serum samples was 0. 03 - 50. 00 μg · mL- t, coefficient of recoveries of low, medium and high concentrations in two groups （ group A ： 3.41, 6. 82, 27.27 μg· mL-1 ; group B ： 0. 05, O. 21, 0. 85 μg · mL- 1 ） were higher than 97%. Inter day and inner day RSD were less than 5%. Conclusion This method has good accuracy, high sensitivity, convenient opera- tion. It can fully satisfy the requirement of concentration monitoring of methotrexate in the pediatric clinical practice.

关 键 词：甲氨蝶呤 固相萃取 高效液相色谱法 

分 类 号：R979.1[医药卫生—药品] R969.1[医药卫生—药学]