重楼生化汤治疗剖宫产术后恶露的疗效及对子宫内膜MMP-9、TIMP-1表达的影响  

Study of efficacy of Chonglou Shenghua Tang for lochia and the expression of MMP- 9、TIMP- 1 in endometrium after caesarian section

作　　者：江延姣[1] 叶慧君[1] 刘佳俐[1] 赵再秋[1] 

机构地区：[1]浙江中医药大学附属第二医院,浙江杭州310005

出　　处：《浙江实用医学》2014年第2期87-89,92,共4页Zhejiang Practical Medicine

基　　金：浙江中医药管理局科研项目(2012ZB068);浙江中医药大学校级科研项目(2011ZY15)

摘　　要：目的观察重楼生化汤治疗剖宫产术后恶露的疗效及对子宫内膜基质金属蛋白酶-9(MMP-9)及其基质金属蛋白酶抑制物-1(TIMP-1)表达的影响。方法选择符合纳入标准及排除标准的剖宫产术后产妇90例,随机分成3组,其中重楼生化汤组、经典生化汤组及安慰剂组各30例,术后6小时起分别给予重楼生化汤、经典生化汤、安慰剂治疗,各100mL,2次/d,连服14天。观察恶露的量及持续时间,并用免疫组织化学法监测服药前及服药后第16天各组子宫内膜组织MMP-9和TIMP-1的表达。结果恶露量及持续时间,恶露持续时间重楼生化汤组均显著优于经典生化汤组及安慰剂组(均P<0.05);子宫内膜MMP-9和TIMP-1的表达,治疗前三组MMP-9、TIMP-1与MMP-9/TIMP-1经方差分析差异无统计学意义(P>0.05),治疗后三组MMP-9表达的组织学积分从低到高依次为:重楼生化汤组、经典生化汤组、安慰剂组。重楼生化汤组显著低于经典生化汤组和安慰剂组(均P<0.05),经典生化汤组显著低于安慰剂组(P<0.05)。三组TIMP-1表达的组织学积分从高到低依次为:重楼生化汤组、经典生化汤组、安慰剂组。重楼生化汤组显著高于经典生化汤组和安慰剂组(均P<0.05),经典生化汤组与安慰剂组比较差异无统计学意义(P>0.05)。三组MMP-9/TIMP-1比值从低到高依次为:重楼生化汤组、经典生化汤组、安慰剂组,各组间比较差异均有统计学意义(均P<0.05)。结论剖宫产术后服用重楼生化汤可以减少恶露量及持续时间;并可以促进子宫内膜的修复。Objective To survey the efficacy of Chonglou Shenghua Tang for lochia ,and the expression of matrix metalloproteinases -9 （MMP-9 ） and tissue inhibitor of metalloproteinase -1 （TIMP-1 ） in endometrium after caesarian section .Methods 90 women underwent caesarian section（CS） according with inclusion criteria and exclusion criteria randomly divided into 3 groups ,including Chonglou Shenghua Tang group（30 cases） ,Shenghua Tang group（30 cases） and placebo group （30 cases） ,and were administered by Chonglou Shenghua Tang , Classical Shenghua Tang and placebo 100 mL ,per 12 hours for 14 days after CS respectively .the amount and duration of lochia rubra ,dura-tion of lochia were observed ,and the expression of MMP -9 and TIMP-1 in endometrium were determined using immunohistochemistry at 16th days after CS .Results The amount and duration of lochia rubra were statistically significant differences between the three groups , Chonglou Shenghua Tang group was significantly superior to the classical Shenghua Tang group and placebo group （ P〈0.05） .The levels of MMP-9、TIMP-1 expression were no significant differences between groups before treatment .The levels of MMP -9 expression decreased gradually in Chonglou Shenghua Tang group ,Classical Shenghua Tang group and placebo group at 16th days after CS ,respectively ,with statisti-cal significance（ P〈0.05） .The levels of TIMP-9 expression and the ratio of MMP -9/TIMP-1 increased gradually in Chonglou Shenghua Tang group ,Classical Shenghua Tang group and placebo group at 16th days after CS respectively ,with statistical significance （ P〈0.05 ） . Conclusion Taking Chonglou Shenghua Tang can reduce the amount and duration of lochia ,and can promote the repair of the endometrium .

关 键 词：剖宫产 恶露 重楼生化汤 MMP-9 TIMP-1 

分 类 号：R271.9[医药卫生—中西医结合]