培养条件对眼点拟微绿球藻油脂含量和EPA产率的影响  

The Effect of Culture Conditions on the Lipid Content and Epa Productivity of Nannochloropis Oculata

作　　者：卢美贞[1] 沈林杰[1] 陆向红[1] 窦晓[1] 计建炳[1] 

机构地区：[1]浙江省生物燃料利用技术研究重点实验室浙江工业大学化学工程与材料学院,杭州310014

出　　处：《中国粮油学报》2014年第5期80-83,89,共5页Journal of the Chinese Cereals and Oils Association

基　　金：浙江省重大科技专项(2006C11015);浙江省教育厅项目(Y201224695)

摘　　要：眼点拟微绿球藻（N.oculata）具有生长速度快、含油量高等优点,且其脂肪酸组成中含有大量二十碳五烯酸（EPA）,因此它是微藻生物柴油的良好原料.以眼点拟微绿球藻为对象,研究了培养时间、盐度、pH值等培养条件对眼点拟微绿球藻油脂含量和EPA产率的影响.试验表明：选择稳定5d为眼点拟微绿球藻的最佳采收时间,油脂含量为378.1 mg/g干藻,EPA产率为5.5 mg/（L·d）;当盐度为16.7时,眼点拟微绿球藻的油脂含量和EPA产率均能达到最高,油脂含量为506.0 mg/g干藻,EPA产率为7.37 mg/（L·d）;在适合眼点拟微绿球藻生长的pH值（8.0～10.0）下,保持整个培养过程中pH值为8.0i油脂含量和EPA产率均较高,油脂含量为471.7 mg/g干藻,EPA产率为7.45 mg/（L·d）.The Nannochloropis oculata （N. oculata） is one of the favorable materials for microalgae biodiesel, which grows well and has high lipid content. Meanwhile, it~ rich in eicosapentaenoic acid （EPA）. In the study we- have taken the N. oculata as the research object to study the effects of the cultural condition such as the salinity, the medium pH, culture time on the lipid contents, and EPA productivity. The results of this experiment showed that： The optimum collecting time was when the N. oculata cells had stayed in the stationary phase for five days, with the lipid content of 378.1 mg/g dry algae and the EPA productivity of 5.5 mg/（ L ~ d）. Both of the lipid contents and EPA productivity reached the highest when the salinity was 16.7 with lipid content of 506.0 mg/g dry algae and the EPA productivity of 7.37 mg/（ L ·d）. On the condition of pH of 8.0 - 10.0 which was suitable for N. oculata grow- ing, while keeping the pH of 8.0 during the culture process, both of the lipid content and EPA productivity reached much more higher with lipid content of 471.7 mg/g dry algae and the EPA productivity of 7.45 mg/L · d.

关 键 词：培养时间 盐度 PH值 眼点拟微绿球藻 油脂含量 EPA产率 

分 类 号：TQ646.4[化学工程—精细化工]