SF/COL/PLCL静电纺丝三维纳米纤维支架材料的性能研究  

A research on the performance of SF/COL/PLCL electrospun three-dimensiona nanofiber scaffold

作　　者：殷丽华[1] 王红[1] 张忠芮 黄嵩涛 王家奎[1] 秦子顺[1] 孙琪[1] 余占海[1] 

机构地区：[1]兰州大学口腔医学院,兰州730000

出　　处：《功能材料》2014年第8期8102-8107,共6页Journal of Functional Materials

基　　金：国家自然科学基金青年科学基金资助项目(81102712);甘肃省中医药科研课题资助项目(GZK-2012-47)

摘　　要：采用静电纺丝法以六氟异丙醇(HFIP)为溶剂,将质量比为70∶30的SF/COL(丝素/胶原)和PLLC(聚左旋乳酸己内酯)共混制备质量比为0∶100,30∶70,50∶50,70∶30,100∶0的8%SF/COL/PLCL的三维纳米纤维支架,25%戊二醛蒸汽交联48h后检测各组比例支架材料的理化性能及生物性能。扫描电镜显示纳米纤维形貌良好,均为纳米级别;X射线衍射结果显示交联后材料结晶度增强,SilkⅠ构象由α-折叠形成稳定的β-折叠;材料交联后为疏水性,PLCL的加入明显改善材料的亲水性能;热稳定性随着PLCL浓度的增加也愈加稳定,交联后尤其明显。细胞培养实验证明,随着培养时间延长,细胞在不同比例的支架上粘附、铺展和增殖均良好,但hPDLSCs与配比为30∶70的SF/COL/PLCL支架复合后增殖速率最快。The aim of this study was tostructure a kind of three-dimensiona nanofiber scaffold composed of SF, collagenⅠand PLCL by using electrospinning technique and to evaluate its performance.We took 1,1,1,3,3,3-hexafluoro-2-propanol（HFIP）as solvent to prepare different mass ratios of SF/collagenⅠ/PLCL scaffolds（0∶100,30∶70,50∶50,70∶30,100∶0）and the properties of these scaffolds were studied before and after they were crosslinked by 25% glutaraldehyde vapor for 48hours.The results were described as follow：scanning electron microscopy（SEM）and the contact water angle meter analysis showed that the uniformity of composite nanofibers was improved and with the existence of PLCL,the hydrophilic property decreased.XRD analysis indicates that the nanofibers scaffolds had higher crystallinity after crosslinked.The thermal stability of the scaffold material was also increased.With rising levels of PLCL,the properties of the scaffolds were increased significantly after crosslinking.When the human periodontal ligament stem cells（hPDLSCs）were seeded on SF/collagenⅠ/PLCL scaffolds,they grew well and showed better ability of adhesion and proliferation.Among the scaffolds,PLCL content of 70% did best with significant differences.Our study indicated that this kind of electrospun three-dimensiona nanofiber scaffold might be a potential candidate for restoration of periodontal defect and the periodontal tissue engineering.

关 键 词：静电纺丝 复合三维纳米支架 聚左旋乳酸己内酯 理化性能 生物学性能 

分 类 号：TQ340[化学工程—化纤工业]