住院新生儿大肠杆菌分离株脉冲电场凝胶电泳分型  

PFGE typing of escherichia coli isolated from hospitalised neonates

作　　者：丁翊君[1] 王亚娟[1] 姚开虎[2] 高薇[2] 董方[3] 俞桑洁[2] 王慧欣[1] 

机构地区：[1]首都医科大学附属北京儿童医院新生儿中心 [2]首都医科大学附属北京儿童医院微生物免疫室 [3]首都医科大学附属北京儿童医院检验科细菌室

出　　处：《中国实用儿科杂志》2014年第5期373-375,共3页Chinese Journal of Practical Pediatrics

基　　金：首都医学发展科研基金(2009-2078);北京市卫生系统高层次卫生技术人才培养计划(2009-3-4)

摘　　要：目的通过对新生儿临床分离的大肠杆菌菌株进行分型以示大肠杆菌菌株间的异质性和亲缘性,了解大肠杆菌感染的分子流行病学特征。方法对2009年9月至2012年5月间北京儿童医院新生儿中心住院的102例患儿的110株大肠杆菌进行脉冲场凝胶电泳分型（PFGE）。结果 110株大肠杆菌临床分离株经PFGE共分65型,相似系数在47.3%～100%,条带在13～22条。优势基因型有13株,相似系数在80.4%～100%。有8对临床分离菌株分别来自于同一患儿,其中7对经PFGE分型方法证实它们属于同一型别（其中1对菌株来自于同一患儿的血液及脑脊液的标本,经PFGE分型证实属于同一基因型,相似系数为93.8%）;另1对显示不是同一型别,亲缘关系较远,故从同源性分析来看不除外不同菌株感染或污染的可能。5株菌株经PFGE证实菌株间亲缘关系较近,其中1株分离株来源的患儿曾与4株分离株来源的患儿同住一病房,考虑为院内获得性感染。结论 PFGE分型方法准确、可靠,可以协助临床明确和区分致病株与非致病株,追踪传染源,对于院内感染的防控起到重要的作用。Objective To investigate the molecular epidemiology of Escherichia coli isolated from neonates in BejingChildren＇s Hospital.MethodsPulsed field gel electrophoresis（PFGE）typing was conducted for 110 strains of Escherichia coli clinical isolates from102 neonates hospitalized at the Neonatal Center of Beijing Children＇s Hospital from September 2009 to May 2012.ResultsTotally 110 strains of Escherichia coli isolated by PFGE typing were divided into65 types,the similarity coefficient between 47.3%～100%.Dominant gene type contained 13 stains,similarity coefficient between 80.4%～100%. There were 8 pairs of clinical isolates strains from the same neonatal patient,7 pairs of whichwere confirmed by PFGE typing methods that they belonged to the same type（1 of which isolated from the same neonatalpatient with cerebrospinal fluid and blood,belonging to the same genotype,the similarity coefficient being 93.8%）. The other 1 pair of strains,typed by PFGE method,showed that they were not the same type and were distantly related. Itmeant that they were different strains infection or contamination. Five of strains by PFGE typing confirmed that geneticrelationship was close,and 1 of them had ever shared the same ward with the other 4 strains.Conclusion PFGE technique is accurate and reliable;PFGE study is helpful for clinicians to confer and distinguish the pathogenic strains,and trace the source of infection.It plays an important role in preventing the occurrence of nosocomial infections.

关 键 词：大肠杆菌 电泳 凝胶 脉冲场 新生儿 

分 类 号：R72[医药卫生—儿科]