垃圾焚烧区周边居民血浆中细胞因子的表达水平及其影响因素  

Plasma Levels of Cytokines and the Risk Factors in Residents Living in the Vicinity of an Watse Incinerator

作　　者：霍倩[1] 张晨[1] 于茂辉 周志俊[1] 吴庆[1] 

机构地区：[1]复旦大学公共卫生学院、公共卫生安全教育部重点实验室,上海200032

出　　处：《环境与职业医学》2014年第5期336-341,共6页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金项目(编号:21277033);国家高技术研究发展计划(863计划)(编号:2012AA062802)

摘　　要：[目的]探讨垃圾焚烧区周边居民外周血中细胞色素P450 1A1(CYP1A1)基因mRNA表达水平和血浆中细胞因子白介素(IL)-6、IL-17A、IL-17F、IL-21、IL-23的表达水平。[方法]选择居住社区位于垃圾焚烧厂烟囱下风向<10 km范围内某社区的男性居民42名为暴露组;居住区距垃圾焚烧区>10 km且无明显环境接触垃圾焚烧污染物的44名男性居民为对照组。采用半定量逆转录-聚合酶链反应检测研究对象外周血中CYP1A1基因mRNA相对表达水平;Bio-PlexTM悬液芯片测定研究对象血浆中IL-6、IL-17A、IL-17F、IL-21、IL-23细胞因子的浓度。[结果]暴露组人群外周血CYP1A1基因mRNA相对表达水平高于对照组人群的平均水平(P<0.05)。暴露组人群血浆中细胞因子IL-17F、IL-21、IL-23的浓度均高于对照组(P<0.05)。细胞因子IL-17F、IL-21、IL-23的浓度经自然对数转换后分别与暴露、年龄、体质指数等因素做多元线性回归分析,发现暴露与IL-17F、IL-23表达水平的升高有关(P<0.05)。[结论]暴露组人群外周血CYP1A1基因mRNA表达水平高于对照组人群的平均水平,且两组人群血浆中IL-17F、IL-23的表达水平亦有差异。[ Objective ] To measure the levels of a panel of cytokines [interleukin-6（IL-6）, IL-17A, IL-17F, IL-21, IL-23] in plasma and the expression of cytochrome P450 1AI（CYPIA1） gene in peripheral leukocytes of residents living around an waste incinerator. [ Methods ] This present study recruited 42 male residents from a community within 10 km downwind from the waste incinerator （exposure group） and 44 male residents from a community beyond 10 km from the waste incinerator and without known industrial pollution sources （control group）. CYPIA1 gene expressions in participants＇ leukoeytes were determined with semi-quantitative reverse transcriptase polymerase chain reaction, and plasma cytokine （IL-6, IL-17A, IL-17F, IL-21, IL-23） levels were measured by Bio-PlexTM Cytokine Panel. [ Results ] The relative CYPIA1 gene expression levels in the exposure group were significantly higher than those in the control group （P 〈 0.05）. Also, the plasma levels of IL-17F, IL-21, and IL-23 in the exposure group were significantly higher （P〈0.05）. After adjusting for age, body mass index, smoking, and alcohol consumption, the transformed levels of IL-17F and IL-23 were associated with exposure （P 〈 0.05） in the multi linear regression analyses. [ Conclusion ] The mRNA expressions of CYPIA1 and the plasma levels of IL-17F and IL-23 in residents living in the vicinity of the waste incinerator are remarkably hizher than those of the residents living further awav from the waste incinerator.

关 键 词：垃圾焚烧 环境污染物 白介素-17F 白介素-23 细胞因子 血浆 

分 类 号：R126.8[医药卫生—环境卫生学]