Analysis of colonic autofluorescence spectra using multivariate curve resolution alternating least squares  

作　　者：刘丽娜 杨常顺 李伟华 李步洪 谢树森 

机构地区：[1]Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education,Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University [2]Department of Surgical Oncology, Fujian Provincial Hospital

出　　处：《Chinese Optics Letters》2014年第5期57-60,共4页中国光学快报（英文版）

基　　金：supported by the Fujian Provincial Science and Technology Foundation(No.2009Y0015);the Program for New Century Excellent Talents in University of China(No.NCET-10-0012);the Program for Changjiang Scholars and Innovative Research Team inUniversity(No.IRT1115)

摘　　要：Autofluorescence （AF） spectra of colonic normal and adenocarcinoma tissues are measured under excitation of 337 nm and analyzed by multivariate curve resolution alternating least squares （MCR-ALS） method using non-negativity constraint. Collagen, nicotinamide adenine dinucleotide hydrate （NADH） and elastin are identified as the main contributing biomedical components. Fisher＇s discrminant anlysis （FDA） on the concentration profiles of the principle components （PCs） shows acceptable sensitivity, specificty and accuracy for discrminanting the adenocarcinoma tissues from the normal tissues. MCR-ALS is a powerful tool for characterzing the spectra profiles of the main biochemical components in neoplasm transformation.Autofluorescence （AF） spectra of colonic normal and adenocarcinoma tissues are measured under excitation of 337 nm and analyzed by multivariate curve resolution alternating least squares （MCR-ALS） method using non-negativity constraint. Collagen, nicotinamide adenine dinucleotide hydrate （NADH） and elastin are identified as the main contributing biomedical components. Fisher＇s discrminant anlysis （FDA） on the concentration profiles of the principle components （PCs） shows acceptable sensitivity, specificty and accuracy for discrminanting the adenocarcinoma tissues from the normal tissues. MCR-ALS is a powerful tool for characterzing the spectra profiles of the main biochemical components in neoplasm transformation.

关 键 词：HISTOLOGY HYDRATES TISSUE Tissue engineering 

分 类 号：O433[机械工程—光学工程]