氯喹对AHNP肝损伤Toll样受体2/4表达的影响及作用机制研究  

Effect of chloroquine on the expression of liver injuy AHNP Toll-like receptor 2/4 and its mechanism

作　　者：刘娟[1] 谭锋[2] 欧阳明[2] 

机构地区：[1]长沙医学院公共卫生系,长沙410219 [2]长沙医学院外科教研室,长沙410219

出　　处：《中南药学》2014年第4期350-353,共4页Central South Pharmacy

基　　金：湖南省教育厅科学研究项目(No.08C151)

摘　　要：目的探讨氯喹对急性出血坏死性胰腺炎(AHNP)肝损伤中促炎因子及Toll样受体2/4(TLR2/4)mRNA表达作用机制的影响。方法采用逆行胆碱牛黄胆酸钠(TAC)逆性建立AHNP肝损伤动脉模型,将36只健康成年雄性SD大鼠随机分为假手术组(NC组)、胰腺炎组(SAP组)以及氯喹治疗组(治疗组),每组12只。采用RT-PCR法测定各组大鼠不同时间点肝损伤TLR2/4 mRNA表达情况。结果 SAP组同一时间点肝腹水、胰腺病理组织评分及肝病理组织评分均高于NC组,NO水平低于NC组,差异有统计学意义(P<0.05)。治疗组血清淀粉酶、ALT、AST、TNF-α水平显著低于SAP组,NO高于SAP组(P<0.05)。SAP组3 h TLR2、TLR4水平、肝腹水、胰腺病理组织评分、肝病理组织评分显著高于治疗组,且在12 h达到最高峰。结论 AHNP肝损伤的发生及进展可能与TLR2/4 mRNA表达上调有关,氯喹可显著抑制AHNP肝组织中TLR2/4 mRNA的表达,减轻肝损伤。Objective To investigate the expression of of liver injuy AHNP Toll-like receptor 2/4 and its mechanism. Methods The AHNP liver injury rat model were established by retrograde chorines sodium taurocholate（TAC）. A total of 36 healthy adult male SD rats were randomly divided into sham group（NC group）, pancreatitis（SAP group） and chloroquine group（treatment group）. TLR2/4 mRNA expression of each group rats at different time points were determined by RT-PCR. Results The levels of TLR2, TLR4, hepatic ascites, pancreatic histological score and liver tissue score of the SAP group were higher than NC group, while levels of NO lower than the NC group（P〈0.05）. The levels of serum amylase, ALT, AST, TNF-α, hepatic ascites, pancreatic histological score and liver tissue score of the treatment group at 3 h were significantly lower than the same experiment time of the SAP group（P〈0.05）, and peaked at 12 h. Conclusion AHNP onset and progression of liver injury may be associated with upregulation TLR2/4 mRNA, chloroquine significantly inhibited AHNP TLR2/4 mRNA expression in liver injury, reduce liver injury.

关 键 词：氯喹 胰腺炎 TOLL样受体 

分 类 号：R965[医药卫生—药理学]