牛源犬新孢子虫IMP1基因的克隆与序列分析  

Cloning and Sequence Analysis of Bovine Neospora caninum IMP1 Gene

作　　者：张蕾[1] 贾立军[1] 鲁承[1] 

机构地区：[1]延边大学农学院动物医学系,吉林延吉133002

出　　处：《安徽农业科学》2014年第15期4566-4568,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金(31160501;31360605);吉林省重点科技攻关项目(20140204078NY);吉林省青年科研基金(201201076)

摘　　要：[目的]为了解牛源犬新孢子虫IMP1基因生物学特性,分析牛源犬新孢子虫IMP1基因的克隆与序列。[方法]应用PCR技术扩增IMP1基因,将纯化的PCR产物和pMD18-T Simple Vector连接,构建pMD18-IMP1重组克隆质粒,进行PCR鉴定和酶切鉴定,将鉴定为阳性的质粒进行测序分析。[结果]PCR扩增犬新孢子虫IMP1基因大小为762 bp,克隆质粒经测序分析与GenBank(XM_003879531.1)中IMP1基因的同源性为99.9%。[结论]该试验为犬新孢子虫IMP1基因的表达及后续研究奠定了基础。[Objective]In order to understand IMP1 genitival biological characteristics,analyze cloning and sequence of bovine Neospora caninum IMP1 gene. [Method] PCR technology was applied for amplification of IMP1 genes. The products of purified PCR were connected with pMD18-T Simple Vector to construct the pMD18-IMP1 cloned plasmid,then PCR identification and enzyme digestion identification of recombinant plasmid were conducted. The positive plasmid was identified by sequencing analysis. [Result]Neospora caninum IMP1 amplified by PCR is 762 bp,the analysis of sequencing results shows that the GenBank（ XM_003879531. 1） IMP1 is 99% in gene homology. [Conclusion] The method laid a foundation for expression and subsequent research of Neospora caninum IMP1 gene.

关 键 词：犬新孢子虫 IMP1基因 克隆 序列分析 

分 类 号：S188[农业科学—农业基础科学]