2009甲型流感病毒BJ501介导小鼠肺炎模型的建立  

ESTABLISHMENT OF THE MOUSE PNEUMONIA MODEL CAUSED BY THE 2009 INFLUENZA A VIRUS BJ501

作　　者：薛文仲[1] 刘坤[2] 陈小飞[3] 赵瑞[1] 宋宏彬[1] 张传福[1] 孙岩松[4] 

机构地区：[1]军事医学科学院疾病预防控制所,北京100071 [2]军事医学科学院微生物流行病研究所 [3]解放军第三〇九医院 [4]军事医学科学院科研部

出　　处：《解放军预防医学杂志》2014年第2期104-106,共3页Journal of Preventive Medicine of Chinese People's Liberation Army

基　　金：军队病原体网络化检测技术研究(No.2008ZX10004-008);军队系统细菌性传染病病原谱流行规律及变异研究(No.2009ZX10004-205);A型流感病毒H1N1引发肺部炎性损伤及靶向抑制策略研究(No.81000723);国家863计划资助项目(No.2014AA020516);吉林省人兽共患病预防与控制重点实验室-省部共建国家重点实验室培育基地开放课题基金资助项目(No.2012ZPC)

摘　　要：目的建立2009甲型H1N1流感毒株BJ501介导小鼠的肺炎动物模型,并与鼠肺适应流感PR8毒株进行比较,为研究流感的致病性、宿主的适应性及抗流感病毒药物筛选提供实验基础。方法 6～8周的BALB/c雌性小鼠216只随机分为3组,每组72只,鼻腔分别接种相同体积的甲型流感病毒BJ501、甲型流感病毒PR8（阳性对照）和PBS溶液（阴性对照）后,每隔12 h观察小鼠整体反应并测量体质量,在感染第1、3、5、7、9、11、13天分批宰杀,取肺组织检测肺指数并进行病理观察。结果 BJ501组和PR8组的整体反应、体质量变化、病理变化、肺指数变化符合甲型流感病毒感染肺炎的基本特征,其中PR8组第3-8天体质量显著低于PBS组（P〈0.05）,而BJ501组体质量在接毒后第7天才显著低于PBS组（P〈0.05）,BJ501组在第3天、第4天体质量显著高于PR8组（P〈0.05）；肺指数情况：BJ501组和PR8组分别在第9天和第7天达到峰值（1.88±0.43）,（2.73±0.71）,BJ501组在第3-7天明显低于PR8组（P〈0.05）；病理结果表明,接种病毒组小鼠肺脏出现不同程度的病理改变,PR8组炎症重于BJ501组。结论成功建立2009甲型流感病毒BJ501介导的小鼠肺炎模型,并发现BJ501毒株对肺脏的炎性损伤程度要比PR8毒株略轻。Objective Creating influenza A virus（ H1N1） BJ501 strain mediated pneumonia model in mice provides experimental basis for the study of pathogenicity,host adaptation,and drugs for treatment of influenza. Methods A total of 216 BALB/ c mice,6 ～8 weeks old,were randomly divided into 3 groups： influenza A virus（ H1N1） BJ501 group,influenza A virus（ H1N1） PR8（ positive control） group,and PBS（ negative control） group; and 25 μl of influenza A virus（ H1N1） solution（ containing PR8 or BJ501 6.40×104TCID50/ ml） was nasally inoculated into the mice. In the 13 days after inoculation,body weight were observed every 12 h,lung index and pathology were measured and observed in batches on the 1st,3rd,5th,7th, 9th,11th,13th day. Results The whole clinical manifestations in mouse pneumonia model were similar to the basic characteristics of human influenza A. The body weight in PR8 group was significantly lower than that in control group on the 3rd-8th day（ P〈0. 05）,whereas that in BJ501 group was lower than in control group only on the 7th day（ P〈0. 05）. Moreover,the body weight in BJ501 group was higher than that in PR8 group on the 3th,4th day（ P〈0. 05）. The lung index in BJ501 and PR8 group reached the peak on the 9th and 7th day respectively〔（ 1. 88±0. 43）,（ 2. 73±0. 71） 〕. The pathological change of lung in PR8 group was more severe than that in BJ501 group. Conclusion A mouse pneumonia model was successfully established, and the lung inflammatory injury induced by BJ501 is less severe than that by PR8.

关 键 词：甲型流感病毒 H1N1 小鼠 模型 炎症 

分 类 号：R511.7[医药卫生—内科学] R566.1[医药卫生—临床医学]