利用SSR分子指纹和商品信息构建水稻品种身份证  

Construction of Rice Variety Indentity Using SSR Fingerprint and Commodity Information

作　　者：陆徐忠[1] 倪金龙[1] 李莉[1] 汪秀峰[1] 马卉[1] 张小娟[1,2] 杨剑波[1] 

机构地区：[1]安徽省农业科学院水稻研究所,安徽合肥230031 [2]安徽省农业科学院棉花研究所,安徽安庆246003

出　　处：《作物学报》2014年第5期823-829,共7页Acta Agronomica Sinica

基　　金：国家科技支撑计划项目(2012BAD07B01);国家自然科学基金青年科学基金项目(31101204);安徽省农业科学院院长青年创新基金项目(11B0106)资助

摘　　要：为方便水稻种子质量的追溯和管理,提出基于SSR分子指纹和商品信息构建水稻品种身份证的新思路。在前期研究基础上,进一步确定了由12个水稻SSR标记构成的核心标记组(每条染色体1个),对127份安徽省审定(或主推)水稻品种进行荧光标记分析,构建供试水稻品种分子指纹,在此基础上,再与品种基本商品信息相结合,并进行数字化编码,构建了127份水稻的品种身份证。该身份证号码组成的第1部分为商品码,代表品种的基本商品信息,包括品种类别、品种选育(或审定)的区域和时间等;第2部分为指纹码,代表水稻品种的分子指纹信息;第3部分为补充码(或特异基因识别码),代表品种的特异基因信息。还对127份水稻品种身份证进行了条码表述(一维码和二维码),以便水稻品种种子的身份标识和溯源管理。On the base of the previous research, we selected a set of 12 rice SSR markers （one marker for each chromosome） for increasing convenience of the rice seed quality tracking and management. The fluorescent marker analysis was performed on 127 released and popularized authorized （or main plant） rice varieties in Anhui Province. Then, the rice variety ID was constructed by the SSR fingerprint with corresponded commodity information. The rice variety ID included three components： part 1 was recog- nized as commodity code, which reflected crop species, cultivar, breeding （or approval） region and year; part 2 was recognized as fingerprint code, which contained the variety DNA fingerprint information; part 3 was recognized as complement code （specific gene identification code）, reflecting specific genetic information of cultivars. We also described the rice variety ID in the form of bar code or QR code, which might be favorable for rice seed identification and traceability management.

关 键 词：水稻 SSR荧光标记 毛细管电泳 商品信息 品种身份证 

分 类 号：S511[农业科学—作物学]