基于HGB/PTh/AuNCs无标记癌胚抗原免疫传感器的研究  

A label-free immunosensor for the determination of carcinoembryonic antigen based on HGB /PTh /AuNCs

作　　者：赵锦航[1] 牛小方 郑丽[1] 赵锦芝[1] 葛艳珊 杨云慧[1] 

机构地区：[1]云南师范大学化学化工学院,云南昆明650092

出　　处：《化学研究与应用》2014年第5期652-657,共6页Chemical Research and Application

基　　金：国家自然科学基金项目(21165023)资助

摘　　要：本文研制了一种基于中空石墨烯球（ HGB）/聚硫堇/金纳米笼的无标记型癌胚抗原传感器。将合成的中空石墨烯球修饰于玻碳电极（ GCE）表面,在其上电聚合硫堇形成多孔聚硫堇（ PTh）复合膜后吸附固定金纳米笼,然后将癌胚抗体（ anti-CEA）固定到电极表面,制得无标记型癌胚抗原（ CEA）免疫传感器。当抗体与抗原发生免疫反应时,形成的复合物阻碍了电子传递,从而降低聚硫堇的电催化活性。通过示差脉冲伏安法（ DPV）检测聚硫堇的响应电流信号的降低,实现对CEA的无标记检测。在最优的实验条件下,检测癌胚抗原的线性范围为0．5~80 ng·mL-1,线性相关系数为0．9932,检测限为0．17 ng·mL-1。该免疫传感器可用于临床上CEA的检测。在血清样品中进行了CEA的检测,结果令人满意。In this paper,a label-free immunosensor for carcinoembryonic antigen was developed based on hollow graphene ball/poly-thionine/gold nanocage. Synthesized hollow graphene ball（ HGB） was modified on the surface of glassy carbon electrode（ GCE） fol-lowed by electropolymerizing thionine on it to adsorb gold nanocage. Carcinoembryonic antibody（ anti-CEA） was immobilized on the surface of electrode to construct label-free immunosensor for the determination of carcinoembryonic antigen（CEA）. When anti-CEA binds with CEA antigen,the formed immunocomplex obstructed electron transfer,which decrease the electrocatalysis of PTh. The de-crease of differential pulse voltammetry（ DPV） response of PTh was used to realize label-free detection of carcinoembryonic anti-gen. Under the optimal experimental conditions,the linear range was 0. 5~80 ng·mL-1 with the detection limit of 0. 17 ng·mL-1 , the linear correlation coefficient was 0. 9932. This immunosensor has been used to detect CEA in serum sample,the results were sat-isfactory.

关 键 词：中空石墨烯球 癌胚抗原 聚硫堇( PTh) 金纳米笼( AuNCs) 免疫传感器 

分 类 号：O657.1[理学—分析化学]