藻酸盐凝胶中肝癌HepG2细胞的三维培养  

Three- dimensional culture of hepatocarcinoma HepG2 cells in alginate hydrogel

作　　者：韩云竹[1] 宋鸿[1] 

机构地区：[1]遵义医学院微生物学教研室,贵州遵义563099

出　　处：《遵义医学院学报》2014年第2期215-218,共4页Journal of Zunyi Medical University

基　　金：国家自然科学基金资助项目(NO:81160290)

摘　　要：目的采用藻酸盐凝胶为支架进行肝癌HepG2细胞的体外培养,构建体外肝癌细胞三维培养模型。方法将HepG2细胞接种于藻酸盐凝胶中进行培养,通过倒置显微镜观察、H-E染色、钙黄绿素-碘化丙啶荧光复染及MTT实验,了解藻酸盐凝胶内细胞的生长情况。结果在藻酸盐凝胶中HepG2细胞以细胞团的形式生长,增殖活跃。结论成功建立了肝癌HepG2细胞藻酸盐凝胶培养体系,且该体系有利于HepG2细胞的生长。Objective To establish the alginate three -dimensional culture system for human HepG2 cells. Methods HepG2 cells were encapsulated in algiuate hydrogel for three - dimensional culture. Cell morphology and growth in gel was demonstrated by microscopy and hematoxylin -eosin staining, Calcein -AM/PI staining, and MTF assay, respectively. Results HepG2 cells grew well when cultured in alginate gel. Conclusion The algi- nate three -dimensional culture system for human HepG2 cells was successfully established, and the microenvi- ronment provided by alginate hydrogel can contribute to the proliferation of HepG2 cells.

关 键 词：藻酸盐 三维培养 HEPG2 

分 类 号：Q813.1[生物学—生物工程]