河北省华北落叶松天然群体遗传多样性分析  

Genetic Diversity and Population Structure of Larix principis rupprechtii Mayr in Hebei Province

作　　者：范英明[1] 张登荣 于大德 张炎 董明亮[1] 张鸿景 张金凤[1] 

机构地区：[1]林木育种国家工程实验室/林木、花卉遗传育种教育部重点实验室/国家林业局树木花卉育种与生物工程重点开放实验室/北京林业大学生物科学与技术学院,北京100083 [2]四川省西昌市凉北林业局,西昌615000 [3]河北省林业科学研究院,石家庄050061

出　　处：《植物遗传资源学报》2014年第3期465-471,共7页Journal of Plant Genetic Resources

基　　金：北京市与中央在京高校共建项目(GJ2011-2);国家林业局林业公益性行业专项(201004009)

摘　　要：以来自河北8个群体共312个华北落叶松天然个体为材料,利用10对SSR引物对群体进行扩增,所得数据用于其遗传多样性和遗传结构的研究。结果表明：10对SSR位点共检测到42个位点,平均每个位点有4.2个等位基因,位点的等位基因数（Na）为2～6个。群体平均等位基因数为3.36,Shannon指数（I）平均为0.749,观察杂合度Ho为0.405、期望杂合度He为0.423,来自河北北部的围场台子水和吉字以及兴隆雾灵山的多样性水平最高,南部的阜平群体多样性水平最低。AMOVA分子差异分析显示3%的遗传变异存在于群体间,97%的遗传变异则存在于群体内,基因分化系数Gst仅为0.028,表明华北落叶松在河北的群体分化很低。聚类结果表明河北围场两个群体为一类,蔚县、隆化和涿鹿为一类,阜平和赤城为一类,兴隆雾灵山群体距离其他较远。多样性北部群体较高,南部较低,表明华北落叶松可能的衍化方向是从北向南,印证了北部可能是起源中心的结论。Based on 312 samples of 8 natural Larix principis rupprechtii Mayr populations in Hebei province of North China,10 pairs of SSR primers were utilized to study the genetic diversity and population structure of these populations. A total of 42 alleles were detected,with a mean of 4. 2 bands per primer,and the alleles of each locus（ Na） were 2-6. The mean allele of population was 3. 36,the mean Shannon＇s information index（ I） was 0. 749,and the observed heterozygosity and expected heterozygosity were 0. 405 and 0. 423,respectively. Among the 8 populations,Taizishui and Jizi from Weichang county and Wuling mountain populations which all located in the north of Hebei had the richest genetic diversity,while Fuping population had the poorest one. The AMOVA analysis showed that the percentage variation among the populations was 3%,while the percentage variation within the populations was 97%,and Gstwas only 0. 028,illustrating that the genetic differentiation was quite low. Clustering result showed that the two Weichang populations were clustered into one group,the Yuxian,Longhua,and Zhuolu populations were clustered into another group,and the Fuping population and Chicheng population were clustered into one group. The Wuling population was far from the others. The north population have a higher diversity than south,suggestting thatthe evolution direction was from north to south,and north might be the center of origin.

关 键 词：华北落叶松 SSR分子标记 遗传多样性 群体结构 

分 类 号：S791.22[农业科学—林木遗传育种]