应用SRAP标记构建山药种质资源DNA指纹图谱  

DNA Fingerprint of Dioscorea polystachya Turcz. Germplasm Resources Based on SRAP Marker

作　　者：华树妹 贺佩珍 陈芝华 雷伏贵 曹奕鸯 黄玉仙 黄姗[3] 

机构地区：[1]三明市农业科学研究院,福建沙县365051 [2]福建生物职业技术学院,福州350002 [3]三明市烟草公司沙县分公司,沙县365500

出　　处：《植物遗传资源学报》2014年第3期597-603,共7页Journal of Plant Genetic Resources

基　　金：福建省自然科学基金项目(2012J01421);国家级星火计划项目(2011GA720020);国家公益性行业(农业)科研专项(200903022)

摘　　要：利用30对多态性良好的SRAP引物对90份山药种质资源进行PCR扩增,构建扩增图谱,共扩增到722个位点,多态性位点581个,多态性比例为80.47%,每对引物组合检测多态性位点3～30个,每对引物能鉴别6～51份山药种质资源；采用DNA数据分析软件对扩增出的多态性位点进行分析,构建山药种质资源方框指纹图谱,该图谱清晰地反映出每对引物能扩增的多态位点数、鉴别资源份数及资源具体编号,资源在该引物组合下所能检测得到的多态位点数及所处的具体位置等信息；从10对SRAP引物组合中挑选出的21个多态性位点,根据谱带的有无转化成的1/0字符串编码,形成山药种质资源DNA数字指纹图谱,该图谱可鉴别区分90份山药种质资源中的82份资源。同时这些指纹图谱可为下一步的山药品种鉴定,种质资源评价、利用,分子标记辅助育种及品种权保护提供技术支撑。90 Dioscorea polystachya Turcz. germplasm resources were screened using 30 pairs of polymorphic SRAP primer combinations. The amplification plot contained 722 sites,including 581 polymorphic sites with a proportion of 80. 47 %. Every primer combination could detect 3 to 30 polymorphic sites and each primer pair could identify 6 to 51 resources. DNA data analysis software was adopted to analyze polymorphic sites and build box fingerprint of Dioscorea polystachya Turcz. germplasm. The box fingerprint could clearly reflect the relationship of primer pair,resources,and polymorphic sites. Then,DNA digital fingerprint of Dioscorea polystachya Turcz. germplasm resources was formed according to the 1 /0 string encoding with the presence or absence of bands using 21 polymorphic sites from 10 pairs of SRAP primer combinations. The DNA digital fingerprint could identify 82 Diosc-orea polystachya Turcz. germplasm resources from all the 90 materials. These fingerprints were useful for variety identification and protection,evaluation and utilization of germplasm resources,molecular marker-assisted breeding of Dioscorea polystachya Turcz. in the future.

关 键 词：山药 种质资源 SRAP DNA指纹图谱 

分 类 号：S632.1[农业科学—蔬菜学]