利用SSR标记构建萝卜种质资源分子身份证  

Establishment of the Molecular Identification for Radish Germplasm Using SSR Markers

作　　者：邱杨[1] 李锡香[1] 李清霞[1] 陈亦辰 沈镝[1] 王海平[1] 宋江萍[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所/农业部蔬菜作物基因资源与种质创制北京科学观测实验站,北京100081 [2]北京工业大学应用数理学院,北京100124

出　　处：《植物遗传资源学报》2014年第3期648-654,共7页Journal of Plant Genetic Resources

基　　金：国家科技支撑计划项目(2006BAD13B06-4;2013BAD01B04;2012BAD02B01);"863"课题(2012AA021801);农业部资源保护项目(2012-2130135-28);农业部园艺作物生物学与种质创制重点实验室

摘　　要：为了保护我国特有的优异萝卜资源,促进资源的有效区分和合理利用,保障我国萝卜产业发展,目前需积极开展萝卜种质资源的特异性鉴定和种质识别技术研究。本研究基于SSR分子标记、信息处理和图像处理技术,筛选出22对SSR引物对75份来源和特征不同的代表性萝卜种质进行鉴定,共扩增出153条带,其中多态性条带为87条,平均多态性位点百分率为55.49%,平均每对引物可扩增出6.95条带和3.95条多态性带,有效地显示出每份萝卜种质的特异性。基于最少引物鉴定最多种质的原则,利用MATLAB程序筛选出8对SSR引物,依据8对引物的扩增数据,经过多态性谱带的有序编码转换,构建出75份萝卜种质分子身份证。结果显示利用SSR标记构建萝卜种质分子身份证进行种质资源的鉴定和保护是可行的。In order to protect our unique and excellent radish resources,promote efficient resource discrimination and rational utilization,and protect development of radish industry,it is necessary to carry out the specific verification and germplasm identification technology for radish germplasms. Based on SSR markers,information and image processing technology,153 bands were generated among 75 radish germplasms using 22 pairs of SSR primers in this study,of which 87 bands were polymorphic. The percentage of polymorphic alleles was 55. 49%. On average, the numbers of alleles per SSR locus and polymorphic bands were 6. 95 and 3. 95,respectively. The genetic diversity among these radish germplasms was rich. After raw data processing and filtering by MATLAB,75 radish germplasms could be distinguished and their molecular identities were constructed using 8 pairs of SSR primers. It showed that it was feasible to employ SSR markers to construct the molecular identities for elite germplasm resource and new varieties of radish.

关 键 词：萝卜 分子身份证 SSR 

分 类 号：S631.1[农业科学—蔬菜学]