浙江柿树种质资源遗传多样性的RAPD分析  

Genetic Diversity Analysis of Persimmons( Diospyros kaki L. ) Germplasm Resources in Zhejiang Province Using RAPD Marker

作　　者：程诗明[1] 童敏[1] 康志雄[1] 陈友吾[1] 应尚姣 周长富[3] 

机构地区：[1]浙江省林业科学研究院,杭州310023 [2]浙江省永康市林业推广中心,永康321300 [3]湖南省园艺研究所,长沙410125

出　　处：《植物遗传资源学报》2014年第3期668-672,共5页Journal of Plant Genetic Resources

基　　金：国家科技基础条件平台(2003DEA3N027);浙江省科技计划项目(2012F20012);浙江省森林食品研究重点实验室(2011F10069);浙江省果品农业新品种选育重大科技专项(2012C12904-10)

摘　　要：浙江柿树资源丰富,但品种或类型混杂,为探究浙江省柿树种质资源多样性,本研究利用筛选出的16条随机引物,对7个柿树类型40个单株进行了RAPD分析,共得到156条谱带,其中多态性条带138条,平均多态性位点百分率为87.76%。类型间遗传多样性(Dst)为0.7604,远高于类型内个体间遗传多样性(Hs=0.0603),各类型分化系数(Gst)为0.7604,基因流(Nm)为0.1576。根据各类型间的遗传距离进行聚类分析,结果表明研究的各类型内遗传结构比较一致,而不同类型间分化较为严重,存在较为丰富的遗传多样性,且有一定的的基因交流。今后应收集更全面的柿树种质资源,为资源保存及良种培育打下基础。Persimmon resources are rich in Zhejiang province,but they are complicated between varieties or types. To investigate their genetic diversity,40 persimmon trees belong to 7 types were analyzed by RAPD maker using 16 selected random primers. In total,156 fragments were obtained,of which 138 were polymorphic. Average percentage of polymorphic loci was 87. 76%,genetic diversity index（ Dst） among types was 0. 7604 and outclassed that within type（ Hs） of 0. 0603. The coefficient of differentiation（ Gst） was 0. 7604,and the gene flow was 0. 1576. At last,7 types of persimmons resources were clustered according to the genetic distance. The result showed that genetic structure were basically identical within each type,but much more difference existed among 7 types with some gene communications. Widely collection of persimmons resources should be researched in future,so as to lay a foundation for the conservation and fine variety breeding.

关 键 词：柿树 遗传资源 多样性 RAPD 

分 类 号：S665.2[农业科学—果树学]