评价间接法在癌抗原CA72-4生物参考区间建立的应用价值  

Evaluation of value of indirect method in establishing reference intervals of CA72-4

作　　者：陈政君[1] 沈隽霏[1] 宋斌斌[1] 张春燕 吴炯[1] 王蓓丽[1] 郭玮[1] 潘柏申[1] 

机构地区：[1]复旦大学附属中山医院检验科,上海200032

出　　处：《中华检验医学杂志》2014年第5期368-370,共3页Chinese Journal of Laboratory Medicine

基　　金：国家临床重点检验专科建设项目资助项目;“十二五”国家科技支撑计划课题“中国人群常用临床检验项目参考区间及相关技术支撑体系的建立”资助项目（2012BA137801）

摘　　要：目的使用间接法建立本实验室的CA72-4参考区间。方法回顾性研究。通过中山医院实验室信息系统采集数据库中自2010年1月至2011年12月的2460例体检人群的CA72-4检测结果。对数据进行正态转换后，使用“Stem-and-Leaf＆Box Plots”数学方式对数据进行剔除和人组。筛选人组的数据经过男、女性别组问差异检验和年龄相关性分析后，利用非参数排序法建立CA72-4的参考区间。结果经“Stem-and-Leaf＆Box Plots”计算剔除数据139份，共入组2321份数据。其中男1548例，CA72-4结果为1．7（0．4～18．9）U/ml，女773例，CA72-4结果为1．8（0．2～18．9）U/ml。经统计，男、女不同性别组间CA72-4差异有统计学意义（Z=-2．337，P〈0．05），需根据性别建立不同的CA72-4生物参考区间。男、女各组CA72-4结果与年龄无相关性（r=-0．044、0．041，P值均〉0．05）。CA72-4的生物参考区间分别为男〈8．9U/ml，女〈11．6U/ml。结论使用间接法建立检验项目的生物参考区间是一种比较简便的方法，适用于实验室检验项目参考区间的定期评审和一些无法使用直接法建立参考区间的项目。（手华检验医学杂志，2014，37：368-370）Objective To establish the reference interval for CA72-4 in indirect method. Methods All results for CA72-4 that were stored in our laboratory information system of Zhongshan hospital between Jan. 2010 and Dec. 2012 were included in this study. Outliers were identified and omitted using Stem-and Leaf ＆ Box Plots in SPSS statistical software. The treated data was divided into several groups according to gender and age. Nonparametric rank sum test was used to observe the difference between male and female participants and Spearman correlation analysis was used to examine the correlation between CA72-4 and age. Nonparametric reference intervals for CA72-4 were estimated statistically in two gender sub groups. Results After 139 cases excluded, there were 1 548 cases of male （ Median 1.7, 0. 4 to 18.9） and 773 cases of female （ Median 1.8, 0. 2 to 18.9） . There was a significant difference in serum CA72-4 between male and female participants. No significant difference was found in serum CA72-4 among age sub groups. Indirect reference values for CA72-4 of male and female were respectively 0 to 8.9 U/ml and 0 to 11.6 U/ml. Conclusion Indirect method to establish biological reference interval is a relatively simple and less expensive method under the high rapid development of the hospital information network. It can be used in the periodical review and establishing the reference intervals where the direct method can not be used. （ Chin J Lab Med,2014,37.368-370）

关 键 词：生物参考区间 间接法 CA724 

分 类 号：R446.6[医药卫生—诊断学]