小脑浦肯野细胞动作电位传输保真度在大鼠出生后发育期间的变化  

The Changes of Spike Propagation Fidelity During Postnatal Development in Cerebellar Purkinje Cells

作　　者：杨志来 王晋辉[1,2] 

机构地区：[1]中国科学院生物物理研究所,脑与认知科学国家重点实验室,北京100101 [2]中国科学院大学,北京100049

出　　处：《生物化学与生物物理进展》2014年第5期480-486,共7页Progress In Biochemistry and Biophysics

基　　金：国家重点基础研究发展计划(973)(2013CB531304;2011CB504405);国家自然科学基金(30990261;81171033)资助项目~~

摘　　要：小脑浦肯野细胞的轴突是小脑皮层唯一的传出通路,研究其动作电位传输能力对于了解小脑的生理功能具有重要意义.大鼠在出生后第2周到第3周,小脑浦肯野细胞的形态及功能都有显著变化,产生动作电位的能力明显增强.而轴突上动作电位传输能力的变化还有待研究.运用胞体以及轴突的双电极膜片钳技术,研究了出生8天以及15天的Wistar大鼠小脑浦肯野细胞轴突上动作电位的传输.与8天组相比,15天组大鼠小脑浦肯野细胞轴突上动作电位的传输能力明显增强.后超极化可以增强8天组轴突上动作电位的传输能力.研究表明,随着发育的成熟,动作电位的产生能力以及轴突上动作电位的传输能力同步增强.The axons ofcerebellar Purkinje cells are the sole output of the cerebellar cortex. So it is important to study the spike propagation on the axons of cerebellar Purkinje cells. The morphology and function of cerebellar Purkinje cells mature rapidly in two to three weeks atter birth. And the ability to fire spikes increases along with the development in cerebellar Purkinje cells. Whether the capacity of spike propagation on the axons also increase remains elusive. We studied this hypothesis by double-patch recordings in cerebellar Purkinje cells at PND 8 （postnatal day） and 15. The spike propagation fidelity on the axons is higher at PND 15 than 8. Aiterhyperpolarization（AHP） can increase spike propagation fidelity in cerebellar Purkinje cells at PND 8. Our data indicate that the ability to fire spikes and the capacity of spike propagation on the axons increase correspondingly along with the development.

关 键 词：小脑浦肯野细胞 动作电位 传输 电压门控钠通道 轴突 

分 类 号：Q421[生物学—神经生物学] Q424[生物学—生理学]