复方玄驹胶囊治疗自身免疫性前列腺炎大鼠的实验研究  

Therapeutic efficacy of Compound Xuanju Capsule on autoimmune prostatitis in rats:An experimental study

作　　者：李天赋 吴秋月[1] 李卫巍[1] 张翠[1] 李娜[1] 商学军[1] 夏欣一[1] 许豪勤 黄宇烽[1] 

机构地区：[1]南京军区南京总医院解放军临床检验医学研究所,江苏南京210002 [2]江苏省计划生育科研所,江苏南京210029

出　　处：《中华男科学杂志》2014年第5期442-447,共6页National Journal of Andrology

基　　金：江苏省自然科学基金(BK2011660);江苏省科技厅省级科技专项(BM2013058)~~

摘　　要：目的:研究复方玄驹胶囊对自身免疫性前列腺炎大鼠的治疗效果。方法:健康Wistar大鼠60只,随机分为5组,对照组、低浓度蛋白免疫模型组、低浓度蛋白免疫+复方玄驹胶囊全方治疗组、高浓度蛋白免疫模型组、高浓度蛋白免疫+复方玄驹胶囊全方治疗组。除对照组外,其余4组用高低两种浓度(15 mg/ml及80 mg/ml)的同种大鼠前列腺匀浆蛋白提纯液进行注射造模。造模(30 d)成功后,对照组和模型组生理盐水[1 ml/(200 g·d)]灌胃,治疗组用生理盐水溶解的复方玄驹胶囊全方[0.068 g/(ml·d)]灌胃,随后分别于30、45、60 d分批处死大鼠。用ELISA法检测大鼠血清中的IL-8、IL-10及TNF-α的表达,并观察大鼠前列腺组织病理切片。结果:与模型组相比,经复方玄驹胶囊全方灌胃治疗的高浓度蛋白免疫大鼠血清中IL-8、TNF-α在造模45 d时由(148.54±17.23)pg/ml、(62.14±5.59)pg/ml下降到(100.77±11.08)pg/ml、(32.63±2.91)pg/ml(P<0.05);60 d时由(143.69±17.28)pg/ml、(59.38±5.50)pg/ml降到(95.77±10.53)pg/ml、(29.63±2.66)pg/ml(P<0.05)。低浓度组造模45 d时IL-8、TNF-α亦由(128.47±12.21)pg/ml、(40.43±3.64)pg/ml下降至(111.76±10.07)pg/ml、(35.44±3.17)pg/ml(P<0.05),60 d时由(131.07±10.93)pg/ml、(43.34±3.91)pg/ml降至(97.46±8.75)pg/ml、(30.44±2.75)pg/ml(P<0.05)。高浓度蛋白免疫的治疗组大鼠血清IL-10在造模45 d、60 d分别由(189.14±16.78)pg/ml、(184.14±15.89)pg/ml上升至(230.48±29.96)pg/ml、(248.48±31.03)pg/ml(P<0.05),低浓度组由(223.14±17.87)pg/ml、(224.14±17.93)pg/ml升高至(231.42±23.18)pg/ml、(249.42±24.97)pg/ml(P<0.05)。病理切片示对照组无明显变化;实验组病理切片显示大鼠前列腺组织腺体结构破坏,炎性细胞浸润;治疗组治疗15 d后光镜下观察病变逐步改善,腺腔增大,上皮细胞轻度增生,间质中无明显炎性细胞浸润,可见少量纤维组织。结论:复方玄驹胶囊能降低自身免疫性前列腺炎大鼠前列腺组织�Objective ： To evaluate the therapeutic effect of Compound Xuanju Capsule （CXC） on autoimmune prostatitis in rat models. Methods ： Sixty healthy male Wistar rats were randomly divided into five groups of equal number： blank control, low-concen- tration purified prostate protein （low-conc PPP）, low-conc PPP ＋ CXC treatment, high-concentration PPP （hi-con PPP）, and hi- conc PPP ＋ CXC treatment. Autoimmune prostatitis models were established by intragastric administration of PPP solution at 15 rag/ ml （low concentration） and 80 mg/ml, respectively. At 30 days after modeling, the rats in the blank control and low-conc and hi-conc PPP model groups were treated with normal saline, and those in the other two groups with CXC at a daily dose of 0. 068 g/ml. At 30, 45, and 60 days, all the animals were sacrificed for observation of pathological changes in the prostate tissue and determination of the levels of IL-8, IL-10, and TNF-α in the serum. Results ： Compared with the PPP models, the hi-conc PPP ＋ CXC group showed significantly reduced levels of IL-8 and TNF-α in the serum at 45 days （ [ 148.54 ±17.23] and [62.14 ±5.59] pg/ml vs [ 100.77 ± 11.08] and [32.63 ±2.91] pg/ml, P〈0.05） and at60 days （[143.69±17.28] and [59.38±5.50] pg/ml vs [95.77 ±10.53] and [29.63 ±2.66] pg/ml, P〈0.05）, and so did the low-cone PPP ＋ CXC group at45 days （[128.47 ±12.21] and [40.43 ± 3.64] pg/ml vs [111.76 ±10.07] and [35.44 ±3.17] pg/ml, P 〈0.05） and at 60 days （ [131.07 ±10.93] and [43.34 ±3.91] pg/ml vs [ 97.46 ± 8.75 ] and [ 30.44 ± 2.75 ] pg/ml, P 〈 0. 05 ）. The serum level of IL-10 was remarkably elevated in the hi-cone PPP ＋ CXC group as compared with that of the PPP models at 45 and 60 days （ [ 189.14 ± 16.78] and [ 184.14 ± 15.89] pg/ml vs [230.48 ±29.96] and [248.48 ±31.03] pg/ml, P 〈0.05）, and so was it in low-cone PPP ＋ CXC group （ [223.14 ＋ 17.87] and [ 224.14 ± 17.93 ] pg/ml vs [ 231.42 ± 23.18 ] and [ 249.42 ± 24.97 ] pg/ml, P 〈 0.

关 键 词：复方玄驹胶囊 自身免疫性前列腺炎 大鼠模型 

分 类 号：R285.5[医药卫生—中药学] R277.5[医药卫生—中医学]