初步探讨鸟嘌呤核苷、姜黄素对AD双转基因小鼠学习记忆的影响  

Preliminary discussion on the effects of guanosine,curcumin on learning and memory in AD double transgenic mice

作　　者：樊惠[1,2] 冯慧利[1,2] 党惠子[1,2] 陈晓培[1,2] 任映[1,2] 杨金铎[1,2] 王蓬文[1,2] 

机构地区：[1]国家中医药管理局三级实验室北京中医药大学东直门医院中药药理学实验室 [2]北京中医药大学东直门医院中医内科学教育部和北京市重点实验室,北京100700

出　　处：《中国实验动物学报》2014年第2期32-35,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然基金面上资助项目(No.81073076);教育部高等学校创新团队(IRT0810)

摘　　要：目的通过Morris水迷宫检测学习记忆和记忆保持能力,判断鸟嘌呤核苷、姜黄素对4月龄APPswe/PS1dE9双转基因小鼠认知功能的影响。方法将3月龄APPswe/PS1dE9双转基因小鼠随机分为模型组、盐酸多奈哌齐组0.92 mg/(kg·d)、鸟嘌呤核苷组20 mg/(kg·d)、姜黄素组200 mg/(kg·d)、姜黄素200 mg/(kg·d)和鸟嘌呤核苷组20 mg/(kg·d),每组12只;并以同月龄野生型C57/BL6J小鼠作对照。每天给药1次,连续给药1个月。应用Morris水迷宫进行行为学检测。结果鸟嘌呤核苷、姜黄素对空间探索、定位航行障碍有改善作用,尤其以姜黄素组明显。结论鸟嘌呤核苷、姜黄素能改善APPswe/PS1dE9双转基因小鼠的早期出现的认知障碍。Objective Using Morris water maze test to evaluate the effects of guanosine and curcumin on cognitive function of APPswe/PS1dE9 double transgenic mice .Methods 3-month old APPswe/PS1dE9 dtg mice were randomly di-vided into model group , donepezil HCL group , guanosine group , curcumin group , curcumin and guanosine group （ n=12）, with age-matched Wild C57BL/6J mice of the same genetic background as normal control group .Medication was giv-en once a day for 1 month.Using Morris water maze to test the spatial learning and memory ability of mice .Results Guanosine and curcumin could improve spatial learning and memory disorders of AD mice , particularly in the group of cur-cumin.Conclusion Guanosine and curcumin improve the cognitive ability of APPswe /PS1dE9 double transgenic mice with early cognitive impairment .

关 键 词：鸟嘌呤核苷 姜黄素 APPswe PS1dE9双转基因小鼠 行为学 

分 类 号：Q95-33[生物学—动物学] R285.5[医药卫生—中药学]