七个中药复方对小鼠止咳化痰作用的对比实验  

Experimental comparison of the antitussive and expectorant effects of seven traditional Chinese medicine compounds in mice

作　　者：张晓菊[1] 吴媛[1] 蒋春燕[1] 张超颖[1] 兰新财 

机构地区：[1]金华职业技术学院 [2]金大康动物保健品有限公司,浙江金华321017

出　　处：《中国实验动物学报》2014年第2期66-69,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：浙江省科技厅公益技术研究农业项目(2011C22033)

摘　　要：目的为了筛选出防治猪呼吸道疾病综合征(PRDC)有效的中药复方制剂,对七个具有止咳化痰作用的中药复方制剂进行了对比实验。方法以小鼠为实验动物,用右美沙芬、氯化铵作阳性药,生理盐水作空白对照,采用浓氨水引咳法和气管段酚红分泌法,对七个中药复方制剂的止咳化痰作用进行了观测。结果方7、方5可显著延长小鼠的咳嗽潜伏期(P<0.05),减少5 min内的咳嗽次数(P<0.05),除方4组外,其他中药组的气管酚红排泌量显著低于对照组(P<0.05),并且方7、方5和氯化铵组小鼠的酚红排泌量显著低于其他处理组(P<0.05)。结论七个中药复方组中,方5、方7的止咳化痰作用最显著,可在进一步实验研究后,作为PRDC的治疗药物。Objective In order to screen effective traditional Chinese medicine compounds to prevent and control porcine respiratory disease syndrome （ PRDC） , seven compound preparations of traditional Chinese medicine were tested and to analyze their antitussive and expectorant effects in mice .Methods Two hundred 6-week old ICR mice （ male∶fe-male=1∶1） were used in this study .Dextromethorphan and ammonium chloride were used as positive control drugs , and physiological saline was used as blank control .The antitussive and expectorant effects of the seven Chinese medicine com-pounds （groups 1, 2, 3, 4, 5, 6, 7） were observed by ammonia-induced cough model and tracheal phenol red secretion method in mice .Results The results showed that compounds 7 and 5 significantly prolonged the cough incubation period （P 〈0.05）, and reduced the cough times within 5 min （P 〈0.05）.Except for the group 4, tracheal phenol red excre-tion in the other groups was significantly lower than that of blank control group （P 〈0.05）, and phenol red excretion in the mice of groups 7, 5 and ammonium chloride group was significantly lower than that in other treatment groups （ P 〈0.05）.Conclusions The Chinese medicine compounds 5 and 7 show most evident expectorant effects , and worthy of fur-ther validation of them as a drug in the treatment of porcine respiratory disease syndrome .

关 键 词：中药复方 止咳化痰 浓氨水引咳法 气管段酚红分泌法 小鼠 

分 类 号：Q95-33[生物学—动物学]