大麦β-淀粉酶活性对其种子在干旱胁迫下萌发影响的研究  

The Effect of β-amylase Activity on the Germination of Barley Seeds Under the Drought Stress

作　　者：周元成[1] 董双全[1] 陈爱萍[1] 

机构地区：[1]山西省农业科学院小麦研究所,山西临汾041000

出　　处：《中国农学通报》2014年第9期113-117,共5页Chinese Agricultural Science Bulletin

基　　金：山西省农业科学院育种工程项目"大麦新品种选育"(11YZGC060)

摘　　要：为了鉴选出抗旱性较强的大麦品种,并以期找出β-淀粉酶活性对大麦抗旱性的影响,对β-淀粉酶活性不同的9个啤酒大麦品(系)种进行聚乙二醇(PEG-6000)模拟干旱胁迫,通过对种子发芽率及发芽势的测定来评价参试品种的抗旱性,并对β-淀粉酶活性与不同PEG浓度下的发芽率进行相关分析。结果表明,大麦β-淀粉酶活性与大麦抗旱性在PEG浓度为15%时,在0.01水平上达显著正相关。大麦种子发芽率随着干旱胁迫程度的增加而降低。5%浓度的PEG对大麦种子的萌发影响较小,10%、15%浓度显著抑制大麦种子萌发,20%浓度下,大麦个别品种发芽停止。处理样品间,‘沪麦16’综合表现出较好的抗旱性,其次A7-30,而‘邯98-58’、‘盐95137’为最差。由此得出,10%的PEG干旱胁迫剂浓度可作为大麦发芽期判断大麦抗旱性的适宜胁迫浓度,β-淀粉酶活性可作为大麦抗旱性早期筛选的指标之一。To sort out drought resistance strong barley varieties and in order to find out β-amylase activity influence on barely drought resistance. Under the drought stress simulated with polyethylene glycol（PEG-6000） by nine malting barley of different amylase activity, to evaluate testing varieties of drought resistance based on the determination of seed germination rate and germination potential and to conduct correlation analysis concerning the β-amylase activity and the germination rate under different concentrations of PEG. Results showed that, it was the significant positive correlation at 0.01 level when PEG leve1 was 15%. Barley seed germination rate was declined with the increasing of drought stress. The effect of 5% PEG was less to barley seed germination 10% and 15% PEG significant inhibition barley seed germination. Barley individual varieties stopped germination when PEG leve1 was under 20%. Among process samples：‘Humai16＇showed good drought resistance comprehensively. Secondly, it was Α7-30,‘Han98-58＇and‘Yan95137＇were the worst. 10% of PEG drought stress concentration can be used as appropriate stress concentrations of barley germination period to judge the barley drought resistance, β-amylase activity can be used as one of barley early screening of drought resistance index.

关 键 词：大麦 β-淀粉酶活性 聚乙二醇(PEG-6000) 干旱胁迫 

分 类 号：S512.3[农业科学—作物学] S332.1