包埋固定化Rhodopseudomonas palustris对邻氯苯酚的降解  

Biodegradation of o-chlorophenol by embedded immobilized Rhodopseudomonas palustris

作　　者：董怡华[1,2] 胡筱敏[3] 张玉革[1] 高丹[1] 

机构地区：[1]沈阳大学环境学院,辽宁沈阳110044 [2]沈阳大学区域污染环境生态修复教育部重点实验室,辽宁沈阳110044 [3]东北大学资源与土木工程学院,辽宁沈阳110004

出　　处：《中南大学学报（自然科学版）》2014年第4期1369-1376,共8页Journal of Central South University:Science and Technology

基　　金：国家水体污染控制与治理重大专项(2012ZX07202-004);国家科技支撑计划课题(2012BAD14B04)

摘　　要：以海藻酸钠为包埋载体,活性炭为添加材料,对沼泽红假单胞菌Rhodopseudomonas palustris PSB-1D进行固定化,通过正交试验确定固定化微生物小球的最佳制备条件:活性炭添加量为1%,海藻酸钠质量分数为3%,包埋菌体与包埋材料的质量比为1/20。在最佳条件下,微生物小球培养7 d后对2-氯苯酚(2-CP)的降解率为72.6%。对比研究微生物小球和游离细菌的降酚效果。将微生物小球引入序批式好氧生物反应器(SBR)工艺中,分别研究小球投加量、曝气时间、曝气量对生物反应器降解2-氯苯酚效果的影响。试验结果表明:微生物小球对2-CP的降解率较游离细菌有明显提高。在黑暗好氧条件下,有效容积为5 L的固定化生物反应器对2-CP模拟废水降解处理的最佳稳定工艺条件为:微生物小球投加量为20 g,曝气时间10 h,曝气量为100 L/h。在此条件下,经过连续30个周期的测定,微生物小球对2-CP的平均去除率始终保持在65%左右。In order to enhance degradation efficiency, a strain of o-chlorophenol degrading photosynthetic bacteria, named Rhodopseudomonas palustris PSB-1D, was immobilized with sodium alginate as immobilizing carrier and activated carbon as additive material. The optimal preparation condition of the immobilized cells was determined as follows： 1% of activated carbon, 3% of sodium alginate and 1/20 of the mass ratio of the embedded biomass and investment material. Under the optimal immobilization condition, the removal rate of o-chlorophenol by the immobilized cells is 72.6% after 7 d culture time. The o-chlorophenol degrading efficiency of the immobilized cells and free cells were studied and compared with each other. The immobilized cells were then added to the sequencing batch reactor （SBR） and the effects of reaction parameters（immobilized cells addition, aeration time, aeration rate） on o-chlorophenol degradation efficiency in the reactor were studied. The results indicate that immobilized cells have higher o-chlorophenol degradation ability than free cells. Under the condition of dark aerobic, the optimum technological conditions for the bioreactor with effective volume 5 L are as follows： aeration time 10 h, immobilized cells addition 20 g, and aeration rate 100 L/h. Under the conditions, bioreactor system could treat the o-chlorophenol wastewater effectively and steadily, with the removal rate of 65% or so.

关 键 词：固定化微生物 沼泽红假单胞菌 2-氯苯酚 序批式好氧生物反应器(SBR) 废水处理 

分 类 号：X703.1[环境科学与工程—环境工程]