培养方法对灵芝菌丝体胞内三萜产量和抗肿瘤活性的影响  

Influences of culture methods on the yield and antineoplastic activity of intracellular triterpene of Ganoderma lucidum mycelia

作　　者：骆军鑫[1,2] 张劲松[1] 贾薇[1] 冯娜[1] 杨炎[1] 唐庆九[1] 刘艳芳[1] 章慧[1,2] 

机构地区：[1]农业部南方食用菌资源利用重点实验室国家食用菌工程技术研究中心上海市农业遗传育种重点开放实验室上海市农业科学院食用菌所,上海201403 [2]上海海洋大学,上海201306

出　　处：《上海农业学报》2014年第2期33-37,共5页Acta Agriculturae Shanghai

基　　金：国家支撑计划"珍稀真菌高值利用技术研究与产品开发"项目(2012BAD36B05)资助

摘　　要：采用液体震荡-静置两阶段培养法进行灵芝液体深层发酵培养,与液体震荡培养法相比,灵芝菌丝体胞内总三萜含量提高了1.7倍左右,总三萜产量提高了2.5倍左右。研究表明:在静置过程中,上层为白色菌丝体,下层为黄色菌球;上层菌丝体总三萜含量是下层菌丝体的3倍,总三萜产量是下层菌丝体的4倍。另外在静置阶段采用培养瓶不会影响灵芝菌丝体总三萜含量,且上层菌丝体的生物量提高了50%,从而大大提高了上层菌丝体总三萜产量。高效液相色谱(HPLC)分析表明:上层菌丝体中含有的三萜组分种类比下层菌丝体多;细胞试验表明:上层菌丝体的醇提取物在作用体积浓度为50μg/mL时对肝癌细胞HepG2的抑制率达到62.91%。Compared with the liquid shaking flask culture in deep fermentation of Ganoderma lucidum, the two-stage method of shaking culture and static culture could not only make the mycelium biomass not decrease but also increase the total triterpene content and the total triterpene yield by about 1.7 times and about 2.5 times respectively. It was found that during the static culture the upper stratum was white mycelia, the substratum was yellow pellets, the total triterpene content of the upper mycelia was 2 times higher than that of the lower mycelia,and the total triterpene yield of the upper mycelia was 3 times higher than that of the lower mycelia. Besides,at the static culture stage the used flask had no effect on the total triterpene content of the mycelia but the upper mycelium biomass increased by 50% ,thus greatly improving the total triterpene yield of the upper mycelia. The analysis by HPLC showed that the kinds of intracellular triterpene components of the upper mycelia were more than those of the lower mycelia, and the cell test indicated that when the upper mycelia＇s ethanol extraction was at 50 μg/mL,the rate of inhibiting HepG2 cells reached 62.91%.

关 键 词：灵芝 菌丝体 三萜产量 培养法 抗肿瘤活性 

分 类 号：S567.3[农业科学—中草药栽培]