污泥深度脱水对四环素耐药菌和耐药基因的去除特性  

Removal characteristics of tetracyclines resistant bacteria and tetracycline resistance genes in sludge deep dehydration

作　　者：谭国赣 杜勇立[2] 黄满红[3] 张微[3] 

机构地区：[1]中建五局土木工程有限公司,长沙410004 [2]湖南省交通规划勘察设计院,长沙410008 [3]东华大学环境科学与工程学院,上海201620

出　　处：《上海师范大学学报（自然科学版）》2014年第2期175-179,共5页Journal of Shanghai Normal University(Natural Sciences)

基　　金：国家自然科学基金(21007010);湖南省交通厅科研项目(200908;201105);交通运输部科研项目(010353343290)

摘　　要：研究了四环素耐药菌和四环素抗性基因在污泥深度脱水中的去除特性,分别采用稀释平板涂布法和荧光定量PCR对污泥深度脱水前后四环素耐药菌和4种四环素抗性基因(tetA、tetB、tetM和tetX)进行了测定,分析了污泥脱水前后这些污染物的去除特性.结果表明:污泥深度脱水前后四环素耐药菌和四环素耐药基因均被检出,经污泥深度处理工艺处理后,四环素耐药菌和所测定的四环素耐药基因均得到一定去除,tetA、tetB和tetM的去除率均达到80%以上.论文结果可为耐药菌和耐药基因的污染控制提供参考.In order to investigate the removal characteristics of Tetracyclines Resistant Bacteria（TRB） and Tetracycline Resistance Genes（TRGs） in sludge deep dehydration,Dilution plate coating method and Real-Time Polymerase chain reaction（RT-PCR） were used to detected the numbers of TRB and four tet genes（tetA、tetB、tetM and tetX） abundant before and after sludge dehydration,and then TRB and TRGs removal characteristics before and after sludge deep dehydration were analyzed. Results showed that：both TRB and TRGs were determined before and after sludge deep dehydration. However,the TRB and TRGs were partly removed after the treatment of sludge deep dehydration. tetA,tetB and tetM removal percentages reached more than 80%. The results can be used as reference for the control of TRB and TRGs contamination.

关 键 词：污泥 深度脱水 四环素耐药基因 四环素耐药菌 去除特性 

分 类 号：X506[环境科学与工程—环境工程]