4-苄氧基苯酚与鲱鱼精DNA相互作用的研究  

Study on the Interaction Mode between 4-Benzyloxyphenol and Herring Sperm DNA

作　　者：胡亚敏[1] 鲁俊良 王兴明[1] 杨欢[1] 

机构地区：[1]西南科技大学材料科学与工程学院化学系,四川绵阳621010

出　　处：《中山大学学报（自然科学版）》2014年第3期85-90,共6页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：国家自然科学基金资助项目(21072185)

摘　　要：在pH=7.4的Tris-HCl缓冲溶液中,采用荧光光谱法、黏度法等分析方法研究了4-苄氧基苯酚(PBP)与鲱鱼精DNA的作用方式,用摩尔比法确定了PBP与DNA的结合比为2∶1。通过热力学研究得出,在27℃时PBP与鲱鱼精DNA之间相互作用的结合常数为Kθ300.15K=2.16×105L/mol,热力学函数ΔrHθm=-6.18×104J/mol,ΔrSθm=-1.05×103J/(mol·K),ΔrGmθ300.15K=-3.04×104J/mol,结果表明该反应为焓驱动。综合实验结果确定了PBP与DNA的作用方式为部分嵌插以及静电作用。The mechanism of interaction between 4-Benzyloxyphenol （PBP） and herring sperm DNA was studied using fluorescence spectroscopy and viscosity analysis in a Tris-HCl solution （ pH = 7.4）. Using mole ration method, the mole ration between PBP and DNA was determined to be 2. Using the thermodynamic analysis, the binding constant of PBP interaction with DNA was determined to be Kθ 300.15k= 2. 16 × 10^5 L/mol, and the corresponding thermodynamic parameters were △rHθm = -6. 18 × 10^4 J/ mol, △rSrm= - 1.05 × 10^3J/ （ mol · K） , △rGmθm300.15K=- 3.04 × 10^4 J/tool. The result suggested that enthalpy was the driven force in the reaction. The interaction of PBP and DNA was derived from partial intercalation and electrostatic interaction.

关 键 词：4-苄氧基苯酚 溴化乙锭 作用方式 

分 类 号：O657.31[理学—分析化学]