不同营养元素及其配比对枣花粉萌发与花粉管生长的影响  

Effects of Different Nutrient Elements on Pollen Germination and Tube Growth in Ziziphus jujube Mill.

作　　者：韩志强[1] 袁德义[1] 陈文涛[1] 李承想 魏晶[1] 

机构地区：[1]中南林业科技大学经济林培育与保护教育部重点实验室/经济林育种与栽培国家林业局重点实验室,湖南长沙410004

出　　处：《江西农业大学学报》2014年第2期357-363,共7页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家林业公益性行业科研专项经费项目(201204407)

摘　　要：为研究营养元素N、P、K、Ca、Mg、B、Zn、Mo及其配比对枣花粉萌发与花粉管生长的影响。以“金丝4号”枣花粉为试材，运用琼脂培养基萌发法，对不同营养元素条件下花粉萌发率及花粉管长度进行比较。结果表明：（1） Zn、Ca、Mo、Mg对枣花粉萌发与花粉管生长具有抑制作用，而N、P、K、B则具有明显的促进作用。（2）促进花粉萌发最适宜质量浓度：CO（ NH2）2为0．10～0．15 g/L；KH2 PO4为0．10 g/L；KCl为0．25 g/L；H3 BO3为0．02 g/L，促进花粉管生长最适宜质量浓度：CO（ NH2）2为0．10～0．15 g/L；KH2 PO4为0．10 g/L；KCl为0．20～0．25 g/L；H3BO3为0．015～0．02 g/L。（3）N、P、K、B营养元素配合使用极显著地促进花粉萌发和花粉管生长，其中以0．10 g/L CO（ NH2）2＋0．10 g/L KH2 PO4＋0．25 g/L KCl＋0．015 g/L H3 BO3处理的效果最好，其花粉萌发率和花粉管长度分别为87．45％和1350μm，比CK提高了37．28％和1293．5μm。By using the pollen of Ziziphus jujube Mill.cv.“Jinsi4” as the material,the influence of nutrient elements on pollen germination and tube growth was studied by tissue culture in vitro .The results was as follows：（1）the pollen germination and tube growth were inhibited by Zn ,Ca,Mo and Mg,but enhanced by N,P,K and B;（2） the optimum concentrations of CO （NH2）2,KH2PO4,KCl and H3BO3 which raised pollen germination and tube growth were 0.10~0.15 g/L;0.10 g/L;0.25 g/L;0.02 g/L and 0.10~0.15 g/L;0.10 g/L;0.20~0.25 g/L;0.015~0.02 g/L respectively;（3）the combination of 0.10 g/L CO（NH2）2＋0.10 g/L KH2PO4＋0.25 g/L KCl＋0.015 g/LH3 BO3 had the best result ,in this treatment the pollen germination percentage and Length of pollen tube were 87.45%and 1 350μm,37.28%and 1 293.5μm higher than those of CK .

关 键 词：枣 花粉 营养元素 萌发 

分 类 号：S665.1[农业科学—果树学]