内分泌干扰物对鲤鱼器官细胞膜脂肪酸组分的影响  

The impact of endocrine disruptors on membrane fatty acid composition of carp organs

作　　者：张翔[1] 荆博宇[1] 袁小英[2] 郭栋生[1] 

机构地区：[1]山西大学环境与资源学院,太原030006 [2]山西大学化学化工学院学院,太原030006

出　　处：《环境化学》2014年第5期748-752,共5页Environmental Chemistry

摘　　要：选择雌二醇、双酚A和邻苯二甲酸二丁酯等3种内分泌干扰物以及睾酮对鲤鱼染毒,研究了不同处理对鲤鱼肝胰脏、性腺和脑细胞膜中脂肪酸组成和含量的影响以及腺苷酸环化酶活性的变化.结果表明,内分泌干扰物暴露可使不同组织器官细胞膜中饱和脂肪酸含量升高,不饱和脂肪酸含量降低,不饱和脂肪酸中双键的数目减少;其结果可能会对组织器官细胞膜的结构、功能产生一定的影响.在肝胰脏和性腺中,睾酮暴露和雌二醇暴露在细胞膜饱和脂肪酸含量方面表现为效应相一致;只在不饱和脂肪酸花生四烯酸(AA)和二十二碳六烯酸(DHA)含量方面表现为效应相拮抗.酶活性测定结果表明,影响细胞内脂肪酸代谢进而影响细胞膜结构和功能的机理可能与细胞膜腺苷酸环化酶活性发生改变相关.Three kinds of endocrine disruptors estradiol, bisphenol A, dibutyl phthalate and testosterone were exposed at carp, and the effect of them on the content and composition of fatty acid in cell membrane and adenylate cyclase activity in cell of carp hepatopancreas, gonads and brain had been studied. The result showed that to three organs of carp, the exposure of endocrine disruptors can make the contents of saturated fatty acid increased; unsaturated fatty acid decreased in cell membranes, and make the number of double bonds lowered in unsaturated fatty acid. As a result, the structure and function of cell membranes in organs were possibly affected. In hepatopancreas and gonads, the effect of testosterone exposure was consistent with the effect of estradiol exposure in saturated fatty acid content, but is antagonistic with it in the content of unsaturated fatty acid arachidonic acid and docosahexaenoic acid. The result of enzyme activity assay showed that the mechanism of fatty acid metabolism change for exposure, thereby affecting the structure and function of cell membrane, may be related to the activity altered of adenylate cyclase in cell membrane.

关 键 词：内分泌干扰物 细胞膜 脂肪酸 腺苷酸环化酶 

分 类 号：X503.22[环境科学与工程—环境工程]