RP-HPLC法测定阿魏酸衍生物的含量及其有关物质  

RP-HPLC determination of content of ferulic acid derivative and its related substances

作　　者：陈丽芳[1,2] 杨建云[2] 肖炳坤[2] 黄荣清[2] 

机构地区：[1]江西中医药大学,南昌330004 [2]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《现代仪器与医疗》2014年第3期72-74,共3页Modern Instruments & Medical Treatment

基　　金：国家自然科学基金(NO.81072613);十二五国家重大专项(NO.2012ZX09301003-001-010);国家"重大新药创制"科技重大专项(NO.2013ZX09102-016)

摘　　要：目的 ：建立测定阿魏酸衍生物（BL）含量及其有关物质的高效液相色谱法。方法 ：采用Agilent Eclipse C18（250mm×4.6mm,5μm）色谱柱,流动相为甲醇-0.1%乙酸（42：58）,流速1.0mL·min-1；检测波长228nm；进样量20μL；柱温：40℃。结果 ：在该条件下BL主峰与有关物质得到很好的分离；检测浓度在0.08～0.32mg·mL-1范围内具有良好的线性关系（r=0.9996）；检测限和定量限分别为8ng和36ng。结论 ：该方法准确可靠,灵敏度高,专属性好,可用于BL含量及其有关物质的测定。Objective： To establish an RP-HPLC method for determination of the content of ferulic acid derivative （BL） and its related substances. Methods： Agilent Eclipse C188（250mm×4.6mm,5μm） column was adopted;the mobile phase consisted of methanol-0.1% acetic acid （42：58） at the flow rate of 1.0 mL.min-1;The ultraviolet detection wavelength was set at 228nm;Loaded volume was 20μL;the column temperature was 40℃. Results： Using this method,BL and its related substances were well separated;The standard curve was rectilinear in the range of 0.08mg-mL-1 - 0.32 mg.mL-t （r=0.9996） ;The limit of detection and quantification were 8ng and 36ng. Conclusion： The method appeared to be accurate, reliable, sensitive, specific and can be used for the determination of the content BL and its related substances.

关 键 词：反相高效液相色谱法 阿魏酸衍生物 含量测定 有关物质 

分 类 号：O657.72[理学—分析化学] R284.1[理学—化学]