甘蓝型油菜叶片组织培养体系的优化  

The Optimization of Regeneration System of Rapeseed(Brassica napus L.) Leaf

作　　者：申静静[1] 廖玉才[2] 李和平[1] 

机构地区：[1]华中农业大学生命科学技术学院/麦类作物分子生物技术实验室,武汉430070 [2]华中农业大学植物科学技术学院/麦类作物分子生物技术实验室,武汉430070

出　　处：《湖北农业科学》2014年第2期447-450,共4页Hubei Agricultural Sciences

基　　金：科技部中德合作项目(2009DFA32330);国家"863"计划项目(2007AA100505)

摘　　要：以甘蓝型油菜(Brassica napus L.)叶片为材料,分析了影响油菜叶片离体培养的关键因素———基因型、基本培养基、激素配比及外植体取材时间,优化了油菜叶片组织培养体系。结果表明,最佳分化培养基为MS+4 mg/L 6-BA+0.5 mg/L NAA,分化率达75.2%,成苗率达28.1%;生根培养基为1/2 MS+1 mg/L IBA,生根率达100%。同一品系不同苗龄的外植体不定芽分化率和成苗率均存在显著差异,F4品系苗龄为22 d的叶片可以作为组织培养体系的最佳外植体。Using leaf as explants from Brassica napus and focusing on factors（genotype, basic media, the hormones combinations and seeding age of explants） affecting the leaves regeneration in vitro, an effective regeneration system was established. The results showed that MS＋4 mg / L 6-BA＋0.5 mg / L NAA was best for shooting, with differentiation and regeneration rate of 75.2% and 28.1%, respectively 1 / 2 MS＋1 mg / L IBA was best for rooting, with the rooting rate of 100%. The explants of different seeding age from the same genotype showed great difference of shooting differentiation and regeneration capability. The 22-day leaf of F4 cultivar was the best explant for tissue culture.

关 键 词：油菜(Brassica NAPUS L.) 叶片 组织培养 

分 类 号：S565.4[农业科学—作物学]