山羊皮肤组织SCF基因的克隆与序列分析  

Cloning and Sequence Analysis of SCF Gene in Goat Skin Tissue

作　　者：张军杰[1] 锡建中[1] 周荣艳[1,2] 李祥龙[1,2] 李兰会[1] 陈辉[1] 张振红[1] 赵驻军[1,2] 

机构地区：[1]河北农业大学动物科技学院,河北保定071001 [2]河北省牛羊胚胎工程技术中心,河北保定071001

出　　处：《湖北农业科学》2014年第2期450-452,共3页Hubei Agricultural Sciences

基　　金：河北省自然科学基金项目(C2012204009);河北省教育厅项目(Q2012074)

摘　　要：对山羊SCF基因的结构与序列进行了分析,参考山羊和绵羊SCF基因序列设计引物,利用PCR克隆获得了山羊SCF基因的两个剪接体序列,同时对其进行了生物信息学分析。结果表明,山羊SCF基因分泌型和跨膜型编码区长度分别为825 bp和741 bp,长度差异主要是由外显子6缺失/插入引起的,与绵羊和牛的同源性分别为98%和97%。13个物种的SCF基因的系统发育聚类与动物分类学基本一致。To investigate the structure and sequence of SCF gene in goat, primers were designed based on the sequence of goat and sheep. Two transcript splices were obtained with PCR and cloning and bioinformatics analysis of the sequence was made. The results showed that the length of coding sequence of soluble and transmembrane SCF gene in goat is 825 bp and 741 bp, respectively. The length difference was due to the deletion / insertion of 6th exon. The identity of SCF gene between goat and sheep or cattle was 98% and 97%, respectively. The phylogenetic clusters based on SCF gene sequence of thirteen species were the same as the zootaxy.

关 键 词：山羊 SCF基因 分泌型 跨膜型 

分 类 号：S827[农业科学—畜牧学] Q785[农业科学—畜牧兽医]