喜树内生真菌产喜树碱菌株的筛选及药化研究  

The Screening of Endophytic Fungus Producing Camptothec in Camptotheca cuminata Decne.Strains and the Research of Pharmaceutical Chemistry

作　　者：曹晋静[1] 康冀川[2] 

机构地区：[1]贵阳护理职业学院,贵阳550018 [2]贵州大学,贵阳550025

出　　处：《湖北农业科学》2014年第3期565-568,共4页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(30660002);贵州省优秀科技教育人才省长专项基金项目[黔科教办(200304)]

摘　　要：采用PDA培养基对151株喜树内生真菌进行发酵,未筛选出产喜树碱及其类似物的内生真菌,而采用喜树种子煎汁作为诱导物进行诱导培养,即在PDA培养基中加入了喜树种子的煎汁,结果从151株内生真菌中共筛选到4株能产生喜树碱及其类似物的内生真菌,其中产喜树碱的3株,产10-羟基喜树碱的1株。试验筛选出了能产喜树碱及其类似物的内生真菌。With ordinary PDA media, no camptothecin and similar compounds were found in the 151 endophytic fungi isolates. With the addition of the seed decocting liquid to PDA media, camptothecin and similar compounds were detected in 4 isolates from the 151 endophytic fungi. This research screened endophytic fungal isolates to produce camptothecin and similar compounds which could be potentially used for the camptothecin production in the future.

关 键 词：喜树 喜树碱 内生真菌 

分 类 号：S567[农业科学—中草药栽培]