青海产小叶黑柴胡总皂苷提取工艺的优选  

Extracting Total Saponins from Bupleurum smithii Wolff var. parvifolium Shan et Y. Li in Qinghai

作　　者：张丹[1] 陶燕铎[2] 颜学伟[1] 曹纬国[1,3,4] 王刚[1] 

机构地区：[1]重庆医科大学中医药学院,重庆400016 [2]中国科学院西北高原生物研究所,西宁810000 [3]重庆医科大学中医药研究室,重庆400016 [4]重庆医科大学中医药实验教学中心,重庆400016

出　　处：《湖北农业科学》2014年第5期1139-1141,共3页Hubei Agricultural Sciences

基　　金：国家"十一五"科技支撑计划项目(2007BAI45B00);重庆市卫生局中医药科技项目(2010-1-146)

摘　　要：采用单因素试验与正交试验相结合优化小叶黑柴胡(Bupleurum smithii Wolff var.parvifolium Shan et Y.Li)总皂苷的提取工艺,考察了提取温度、提取溶剂pH、乙醇浓度、料液比(g/mL,下同)、提取次数和提取时间对小叶黑柴胡总皂苷得率的影响。结果表明,小叶黑柴胡总皂苷的较优提取工艺条件为料液比1∶10,乙醇浓度80%,提取次数3次,提取时间1.0 h,提取温度80℃,提取溶剂pH为9。该工艺条件下,小叶黑柴胡总皂苷的得率为2.78%。验证试验表明该工艺稳定可行,可为小叶黑柴胡的深入研究提供参考。The technology of extracting total saponins from Bupleurum smithii Wolff var. parvifolium Shan et Y. Li were optimized by single-factor test and orthogonal design factors such as extraction temperature, pH of solvent, extraction times, ethanol concentration and liquid ratio were investigated. The results showed that the yield of total saponins from Bupleurum smithii Wolff var. parvifolium Shan et Y. Li. was 2.78% when it was extracted three times with each time 1 hour under 80 ℃, ethanol concentration of 80%, solid to liquid ratio of 1∶10（g / mL） and pH of 9. The technology was proved to be reliable and could be used as a reference for utilizing of Bupleurum smithii Wolff var. parvifolium Shan et Y. Li.

关 键 词：小叶黑柴胡(Bupleurum smithii Wolff var.parvifolium Shan ET Y.Li) 总皂苷 提取工艺 正交试验 

分 类 号：R284.2[医药卫生—中药学]