类固醇受体辅活化子-3在烧伤后T淋巴细胞功能抑制中的作用  

Study on the Role of Steroid Receptor Coactivator-3 in the Dysfunction of T Lymphocytes after Burn Injury in Mice

作　　者：李军[1] 林露[1] 汪海洋[1] 冉茂荣[1] 刘英海[1] 安虹[1] 牛洁[1] 王军平[2] 粟永萍[2] 

机构地区：[1]成都军区总医院麻醉科,四川成都610083 [2]第三军医大学创伤,烧伤与复合伤国家重点实验室,重庆400038

出　　处：《四川医学》2014年第5期545-548,共4页Sichuan Medical Journal

基　　金：成都军区总医院院管课题(2011YG-B09);创伤;烧伤与复合伤国家重点实验室开放基金(SKLKF200910)

摘　　要：目的探讨类固醇受体辅活化子（SRC）-3蛋白在严重烧伤后T淋巴细胞功能变化中的作用。方法健康SPF级雌性野生型（SRC-3＋/＋）小鼠、SRC-3基因敲除（SRC-3-/-）小鼠各10只,体质量约20 g,分为SRC-3＋/＋组（S组）和SRC-3-/-组（S-组）,每组分为正常（N）和24h两个时相点,每个时相点各5只。制作15%～20%Ⅲ°烧伤模型,按时脱臼处死小鼠,取股动脉血、脾脏和胸腺,并立即分离血浆和外周血、脾、胸腺淋巴细胞。测定血浆IL-2、可溶性IL-2受体（sIL-2R）表达和T淋巴细胞亚群的分布。结果两组正常小鼠血浆IL-2和sIL-2R水平没有显著差别,烧伤后24h均显著升高（P〈0.01）,但S-组小鼠血浆IL-2显著低于S组（P〈0.01）,而血浆sIL-2R显著高（P〈0.01）;正常情况下,S-组小鼠除脾脏CD8＋高于S组小鼠（P〈0.01）外,外周血、脾脏和胸腺CD3＋、CD4＋及CD4＋/CD8＋比值均不同程度低于S组小鼠（P〈0.05或P〈0.01）。烧伤后24h,两组小鼠外周血、脾脏和胸腺CD3＋、CD4＋及CD4＋/CD8＋比值均不同程度下降（P〈0.05或P〈0.01）,CD8＋显著增高（P〈0.05或P〈0.01）,与S组相比,S-组外周血、脾脏和胸腺CD3＋和CD4＋降低更显著（P〈0.01）,CD4＋/CD8＋比值差别均无统计学意义。结论 SRC-3蛋白可能与严重烧伤后T淋巴细胞免疫功能抑制有关,其蛋白缺失可引起T淋巴细胞亚群稳态的改变。Objective To investigate the effect of steroid receptor coactivator（SRC）-3 on T lymphocyte function after severe burns.Methods 10 female healthy SPF-grade SRC-3＋ / ＋mice and SRC-3-/-mice respectively,20g and are named S group and S-group.Each group was divided into two phase points：normal（N） and 24h,which each of points had 5 mice.The distribution of T lymphocyte subsets of peripheral blood,spleen,thymus,and the levels of IL-2 and soluble IL-2 receptor（sIL-2R） were determined.Results There were no statistical significance between two groups about the levels of plasma IL-2 and sIL-2R under normal condition.They were both increased significantly（P〈0.01） at 24h after burn in two groups,but the plasma IL-2 level was significantly lower in the S-group than that of S group（P〈0.01） and the plasma sIL-2R content was markedly higher in the S-group than that of S group.Under normal condition,CD3＋,CD4＋and the ratio of CD4＋/ CD8＋of peripheral blood,spleen and thymus were lower in the S-group than those of S group（P〈0.05 or P〈0.01）,but spleen CD8＋was higher in the SRC-3-/-group than that of S group（P〈0.01）.After burn,the CD3＋,CD4＋and CD4＋/ CD8＋ratio of peripheral blood,spleen and thymus were both decreased（P〈0.05 or P〈0.01）,the CD8＋were significantly increased（P〈0.05 or P〈0.01）.Compared with the S group,the CD3＋and CD4＋of peripheral blood,spleen and thymus were decreased significantly（P〈0.01）,and there were no significant differences in the ratio of CD4＋/ CD8＋in the S-group.Conclusion The SRC-3 protein might be related to the inhibition of T lymphocyte immune function after severe burns,which its deletion can cause the distribution changes of T lymphocyte subsets.

关 键 词：类固醇受体辅活化子-3 淋巴细胞亚群 IL-2 可溶性白介素-2受体 

分 类 号：R644[医药卫生—外科学]