一种适于玉米灰斑病菌快速扩繁的方法  

Multiplication Method of Cercospora zeae-maydis

作　　者：路媛媛[1] 肖淑芹[1] 赵丽琨[1] 杨宁[1] 薛春生[1] 

机构地区：[1]沈阳农业大学植物保护学院,辽宁沈阳110866

出　　处：《云南农业大学学报（自然科学版）》2014年第3期333-336,共4页Journal of Yunnan Agricultural University:Natural Science

基　　金：教育部科学研究重点项目(211038);辽宁省自然科学基金(201102187)

摘　　要：介绍了一种适于玉米灰斑病菌快速扩繁的方法.该方法通过快速研磨灰斑病菌菌丝,制成研磨液后直接涂抹在培养基上进行培养,从而达到快速扩繁的目的.结果表明,取100 μL均匀涂抹在直径为90 mm的PDA平板上,第7天菌落面积最多可达3.35×103 mm2,占培养皿面积的52.57％;采用本方法获得的灰斑病菌第3天开始产生孢子,4～6d就能够获得大量分生孢子,可达2.365×106个/mL,并且产孢时间缩短.研究结果显示：该方法简便易行,快速高效,污染少,为玉米灰斑病菌的生物学、致病机制等进一步研究奠定了基础.该方法可用于其他生长速度缓慢真菌的快速扩繁.Multiplication method of Cercospora zeae-maydis was introduced in this study.Hyphae were grinded to solution and directly applied on the medium for rapid growth of C.zeae-maydis.The results showed colony area of up to 3.35 × 103 mm2 （52.57%） after 7 d with 100 μL spreading on PDA plates （d =90 mm）.Conidia were produced to 2.365 × 106/mL after 4 ～6 d.These finding indicated that it was rapid,simple,less pollution,and useful tool for studying biological character,pathogenesis of C.zeae-maydis.Multiplication method is suitable for other fungi that are difficult multiplication.

关 键 词：玉米灰斑病菌 菌丝生长 扩繁技术 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]