镧(Ⅲ)-芸香甙配合物与人血清白蛋白作用特征的研究  

Study on the Interaction of La(Ⅲ)-Phytomelinovine Complex and Human Serum Albumin

作　　者：熊泽云[1] 张华新[1] 王洪林[1] 施红[1] 

机构地区：[1]荆楚理工学院化工与药学院,湖北荆门448000

出　　处：《化学与生物工程》2014年第5期24-27,共4页Chemistry & Bioengineering

基　　金：湖北省荆门医药工业技术研究院基础项目;荆楚理工学院科技项目(ZR201108;ZR201317)

摘　　要：合成了稀土镧(Ⅲ)与芸香甙的配合物(LaPH),采用荧光光谱法和圆二色性光谱法研究了LaPH与人血清白蛋白(HSA)的作用特征。结果表明,在人体血液离子强度及pH值条件下,LaPH能与HSA依靠氢键和范德华力发生结合反应,导致HSA内源荧光的猝灭,结合过程可自发进行;计算得到了不同温度下结合反应的平衡常数和热力学参数;圆二色性光谱显示LaPH的介入使HSA的α-螺旋、!-折叠等二级结构发生了一定的转变。The La（Ⅲ）-phytomelinovine complex（LaPH）was synthesized.Its interaction with human serum albumin（HSA）was investigated by fluorescence spectroscopy and circular dichroism（CD）spectroscopy.The results indicated that LaPH could bind to HSA by hydrogen bonds and van der Waals force under simulated psychological conditions in vitro,which occured spontaneously and resulted in the quenching of fluorescence of HSA.The equilibrium constants and thermodynamic parameters were calculated.CD Spectra showed the secondary structures of HSA,includingα-helix,!-strand,etc.were changed in the presence of LaPH.

关 键 词：镧(Ⅲ) 芸香甙 人血清白蛋白 荧光光谱 圆二色性光谱 

分 类 号：O657.3[理学—分析化学]